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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Micrococcus luteus Elongation factor G (fusA), partial CSB-YP009373MTO
CSB-EP009373MTO
CSB-BP009373MTO
CSB-MP009373MTO
CSB-EP009373MTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acyl carrier protein (acpP) CSB-YP015636SKY
CSB-EP015636SKY
CSB-BP015636SKY
CSB-MP015636SKY
CSB-EP015636SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine synthetase (glnA) CSB-YP009553FKZ
CSB-EP009553FKZ
CSB-BP009553FKZ
CSB-MP009553FKZ
CSB-EP009553FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyruvate dehydrogenase E1 component subunit beta (pdhB) CSB-YP017717SKX
CSB-EP017717SKX
CSB-BP017717SKX
CSB-MP017717SKX
CSB-EP017717SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyruvate dehydrogenase E1 component subunit beta (pdhB) CSB-YP017717FKZ
CSB-EP017717FKZ
CSB-BP017717FKZ
CSB-MP017717FKZ
CSB-EP017717FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydrolipoyl dehydrogenase (pdhD) CSB-YP006928SKX
CSB-EP006928SKX
CSB-BP006928SKX
CSB-MP006928SKX
CSB-EP006928SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrolipoyl dehydrogenase (pdhD) CSB-YP006928FKZ
CSB-EP006928FKZ
CSB-BP006928FKZ
CSB-MP006928FKZ
CSB-EP006928FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. citri Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717XAG
CSB-EP009717XAG
CSB-BP009717XAG
CSB-MP009717XAG
CSB-EP009717XAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Lactoylglutathione lyase (gloA) CSB-YP009511NGG
CSB-EP009511NGG
CSB-BP009511NGG
CSB-MP009511NGG
CSB-EP009511NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B N-acylneuraminate cytidylyltransferase (neuA) CSB-YP005601NGG
CSB-EP005601NGG
CSB-BP005601NGG
CSB-MP005601NGG
CSB-EP005601NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Translation initiation factor IF-3 (infC) CSB-YP015166FGP
CSB-EP015166FGP
CSB-BP015166FGP
CSB-MP015166FGP
CSB-EP015166FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl carrier protein (acpP) CSB-YP015636SZB
CSB-EP015636SZB
CSB-BP015636SZB
CSB-MP015636SZB
CSB-EP015636SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Branched-chain-amino-acid aminotransferase (ilvE) CSB-YP002601SXB
CSB-EP002601SXB
CSB-BP002601SXB
CSB-MP002601SXB
CSB-EP002601SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fusA), partial CSB-YP009373SWW
CSB-EP009373SWW
CSB-BP009373SWW
CSB-MP009373SWW
CSB-EP009373SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tufA) CSB-YP025342SWW
CSB-EP025342SWW
CSB-BP025342SWW
CSB-MP025342SWW
CSB-EP025342SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine synthetase (glnA) CSB-YP009553SWW
CSB-EP009553SWW
CSB-BP009553SWW
CSB-MP009553SWW
CSB-EP009553SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutaredoxin-1 (grxA) CSB-YP009521SWW
CSB-EP009521SWW
CSB-BP009521SWW
CSB-MP009521SWW
CSB-EP009521SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Lactoylglutathione lyase (gloA) CSB-YP009511SXB
CSB-EP009511SXB
CSB-BP009511SXB
CSB-MP009511SXB
CSB-EP009511SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Porphobilinogen deaminase (hemC) CSB-YP010524SXB
CSB-EP010524SXB
CSB-BP010524SXB
CSB-MP010524SXB
CSB-EP010524SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidyl-prolyl cis-trans isomerase C (ppiC) CSB-YP018473SWW
CSB-EP018473SWW
CSB-BP018473SWW
CSB-MP018473SWW
CSB-EP018473SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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