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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida 2-oxoisovalerate dehydrogenase subunit alpha (bkdA1) CSB-YP002605FFZ
CSB-EP002605FFZ
CSB-BP002605FFZ
CSB-MP002605FFZ
CSB-EP002605FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (bkdB) CSB-YP006530FFZ
CSB-EP006530FFZ
CSB-BP006530FFZ
CSB-MP006530FFZ
CSB-EP006530FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Homeobox protein Hox-B4 (hoxb4) CSB-YP010664XBE
CSB-EP010664XBE
CSB-BP010664XBE
CSB-MP010664XBE
CSB-EP010664XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio anubis Hemoglobin subunit theta-1 (HBQ1) CSB-YP010159ERI
CSB-EP010159ERI
CSB-BP010159ERI
CSB-MP010159ERI
CSB-EP010159ERI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Torpedo californica 43 kDa receptor-associated protein of the synapse (RAPSN) CSB-YP019337TOT
CSB-EP019337TOT
CSB-BP019337TOT
CSB-MP019337TOT
CSB-EP019337TOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thunnus thynnus Somatotropin (gh) CSB-YP009407TEK
CSB-EP009407TEK
CSB-BP009407TEK
CSB-MP009407TEK
CSB-EP009407TEK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uricase (Uox) CSB-YP025648RA
CSB-BP025648RA
CSB-MP025648RA
CSB-EP025648RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uroporphyrinogen-III synthase (hemD) CSB-YP025679ENV
CSB-EP025679ENV
CSB-BP025679ENV
CSB-MP025679ENV
CSB-EP025679ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-glucose 4-epimerase (galE) CSB-YP009197ENV
CSB-EP009197ENV
CSB-BP009197ENV
CSB-MP009197ENV
CSB-EP009197ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelophylax ridibundus Natriuretic peptides A CSB-YP016020PEN
CSB-EP016020PEN
CSB-BP016020PEN
CSB-MP016020PEN
CSB-EP016020PEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Tubulin beta-3 chain CSB-YP025323CH
CSB-EP025323CH
CSB-BP025323CH
CSB-MP025323CH
CSB-EP025323CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein kinase C epsilon type (Prkce) CSB-YP018704RA
CSB-EP018704RA
CSB-BP018704RA
CSB-MP018704RA
CSB-EP018704RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus PS3 ATP synthase subunit a (atpB), partial CSB-YP015070BBK1
CSB-EP015070BBK1
CSB-BP015070BBK1
CSB-MP015070BBK1
CSB-EP015070BBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus PS3 ATP synthase subunit delta (atpH) CSB-YP002355BBK
CSB-EP002355BBK
CSB-BP002355BBK
CSB-MP002355BBK
CSB-EP002355BBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyprinus carpio Parvalbumin alpha CSB-YP019092EQE
CSB-EP019092EQE
CSB-BP019092EQE
CSB-MP019092EQE
CSB-EP019092EQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Ribonucleoside-diphosphate reductase large subunit (ORF19), partial CSB-YP020518VAP
CSB-EP020518VAP
CSB-BP020518VAP
CSB-MP020518VAP
CSB-EP020518VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Thymidine kinase (ORF36) CSB-YP023577VAP
CSB-EP023577VAP
CSB-BP023577VAP
CSB-MP023577VAP
CSB-EP023577VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Growth-regulated alpha protein (CXCL1) CSB-YP006239DXU
CSB-EP006239DXU
CSB-BP006239DXU
CSB-MP006239DXU
CSB-EP006239DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spermophilus citellus Hemoglobin subunit beta (HBB) CSB-YP010150SJE
CSB-EP010150SJE
CSB-BP010150SJE
CSB-MP010150SJE
CSB-EP010150SJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Elongation factor 2 (EEF2), partial CSB-YP007434DXU
CSB-BP007434DXU
CSB-MP007434DXU
CSB-EP007434DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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