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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat Beta-casein (Csn2) CSB-YP006063RA
CSB-EP006063RA
CSB-BP006063RA
CSB-MP006063RA
CSB-EP006063RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Torpedo californica Acetylcholine receptor subunit beta (CHRNB1), partial CSB-YP005395TOT
CSB-EP005395TOT
CSB-BP005395TOT
CSB-MP005395TOT
CSB-EP005395TOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Acetylcholine receptor subunit delta (CHNRD), partial CSB-YP005399CH
CSB-EP005399CH
CSB-BP005399CH
CSB-MP005399CH
CSB-EP005399CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse Glycophorin-A, partial CSB-YP010074HO1
CSB-EP010074HO1
CSB-BP010074HO1
CSB-MP010074HO1
CSB-EP010074HO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bubalus bubalis Beta-lactoglobulin (LGB) CSB-YP027168BWX
CSB-EP027168BWX
CSB-BP027168BWX
CSB-MP027168BWX
CSB-EP027168BWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serum albumin (Alb) CSB-YP001561RA
CSB-BP001561RA
CSB-MP001561RA
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse Metallothionein-1A CSB-YP015107HO
CSB-EP015107HO
CSB-BP015107HO
CSB-MP015107HO
CSB-EP015107HO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xiphias gladius Osteocalcin (bglap) CSB-YP002682XAR
CSB-EP002682XAR
CSB-BP002682XAR
CSB-MP002682XAR
CSB-EP002682XAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Thymidine kinase (TK), partial CSB-YP023577EFA
CSB-EP023577EFA
CSB-BP023577EFA
CSB-MP023577EFA
CSB-EP023577EFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C10 (C10L) CSB-YP003090VAI
CSB-EP003090VAI
CSB-BP003090VAI
CSB-MP003090VAI
CSB-EP003090VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba NADH-ubiquinone oxidoreductase chain 2 (mt:ND2), partial CSB-YP015077DMR
CSB-EP015077DMR
CSB-BP015077DMR
CSB-MP015077DMR
CSB-EP015077DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078HU1
CSB-EP015078HU1
CSB-BP015078HU1
CSB-MP015078HU1
CSB-EP015078HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NADH-ubiquinone oxidoreductase chain 3 (Mtnd3), partial CSB-YP015078MO1
CSB-EP015078MO1
CSB-BP015078MO1
CSB-MP015078MO1
CSB-EP015078MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NADH-ubiquinone oxidoreductase chain 4L (mt-nd4l), partial CSB-YP015080XBE1
CSB-EP015080XBE1
CSB-BP015080XBE1
CSB-MP015080XBE1
CSB-EP015080XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079EQV
CSB-EP015079EQV
CSB-BP015079EQV
CSB-MP015079EQV
CSB-EP015079EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hylobates lar NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079HXW
CSB-EP015079HXW
CSB-BP015079HXW
CSB-MP015079HXW
CSB-EP015079HXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NADH-ubiquinone oxidoreductase chain 4 (mt-nd4), partial CSB-YP015079XBE
CSB-EP015079XBE
CSB-BP015079XBE
CSB-MP015079XBE
CSB-EP015079XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081HU
CSB-EP015081HU
CSB-BP015081HU
CSB-MP015081HU
CSB-EP015081HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081EXP
CSB-EP015081EXP
CSB-BP015081EXP
CSB-MP015081EXP
CSB-EP015081EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NADH-ubiquinone oxidoreductase chain 5 (Mtnd5), partial CSB-YP015081MO
CSB-EP015081MO
CSB-BP015081MO
CSB-MP015081MO
CSB-EP015081MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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