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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Danio rerio Protein PAT1 homolog 1 (patl1), partial CSB-YP017483DIL
CSB-EP017483DIL
CSB-BP017483DIL
CSB-MP017483DIL
CSB-EP017483DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Peptide chain release factor 1 (prfA) CSB-YP015197BPP
CSB-EP015197BPP
CSB-BP015197BPP
CSB-MP015197BPP
CSB-EP015197BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Malate dehydrogenase (mdh) CSB-YP013623BPP
CSB-EP013623BPP
CSB-BP013623BPP
CSB-MP013623BPP
CSB-EP013623BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Nucleoside diphosphate kinase (ndk) CSB-YP015889BPP
CSB-EP015889BPP
CSB-BP015889BPP
CSB-MP015889BPP
CSB-EP015889BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Translation initiation factor IF-2 (infB), partial CSB-YP015164BPP
CSB-EP015164BPP
CSB-BP015164BPP
CSB-MP015164BPP
CSB-EP015164BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Oligoribonuclease (orn) CSB-YP019586BPP
CSB-EP019586BPP
CSB-BP019586BPP
CSB-MP019586BPP
CSB-EP019586BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris ATP synthase subunit beta (atpD) CSB-YP002350LND
CSB-EP002350LND
CSB-BP002350LND
CSB-MP002350LND
CSB-EP002350LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Centrosomal protein of 78 kDa (cep78), partial CSB-YP005250XBF
CSB-EP005250XBF
CSB-BP005250XBF
CSB-MP005250XBF
CSB-EP005250XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytosolic Fe-S cluster assembly factor narfl (narfl) CSB-YP015455DIL
CSB-EP015455DIL
CSB-BP015455DIL
CSB-MP015455DIL
CSB-EP015455DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse TRMT1-like protein (Trmt1l) CSB-YP003588MO
CSB-EP003588MO
CSB-BP003588MO
CSB-MP003588MO
CSB-EP003588MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Rho GTPase-activating protein 21 (arhgap21), partial CSB-YP002022XBF
CSB-EP002022XBF
CSB-BP002022XBF
CSB-MP002022XBF
CSB-EP002022XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio DIS3-like exonuclease 1 (dis3l), partial CSB-YP006912DIL
CSB-EP006912DIL
CSB-BP006912DIL
CSB-MP006912DIL
CSB-EP006912DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis GTPase-activating protein and VPS9 domain-containing protein 1 (gapvd1), partial CSB-YP009256XBE
CSB-EP009256XBE
CSB-BP009256XBE
CSB-MP009256XBE
CSB-EP009256XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Centrosomal protein of 44 kDa (cep44) CSB-YP012282XBE
CSB-EP012282XBE
CSB-BP012282XBE
CSB-MP012282XBE
CSB-EP012282XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203BPP
CSB-EP001203BPP
CSB-BP001203BPP
CSB-MP001203BPP
CSB-EP001203BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris ATP synthase subunit alpha (atpA) CSB-YP002344LND
CSB-EP002344LND
CSB-BP002344LND
CSB-MP002344LND
CSB-EP002344LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Elongation factor Tu (tuf) CSB-YP025342LND
CSB-EP025342LND
CSB-BP025342LND
CSB-MP025342LND
CSB-EP025342LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Methionyl-tRNA formyltransferase (fmt) CSB-YP015150LND
CSB-EP015150LND
CSB-BP015150LND
CSB-MP015150LND
CSB-EP015150LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Glucose-6-phosphate isomerase (pgi) CSB-YP009717LND
CSB-EP009717LND
CSB-BP009717LND
CSB-MP009717LND
CSB-EP009717LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein NPAT (npat), partial CSB-YP015970XBE
CSB-EP015970XBE
CSB-BP015970XBE
CSB-MP015970XBE
CSB-EP015970XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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