Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Protein spinster homolog 3 (spns3), partial CSB-YP022593DIL
CSB-EP022593DIL
CSB-BP022593DIL
CSB-MP022593DIL
CSB-EP022593DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ateles geoffroyi Osteocalcin (BGLAP) CSB-YP002682AVF
CSB-EP002682AVF
CSB-BP002682AVF
CSB-MP002682AVF
CSB-EP002682AVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lagothrix lagotricha Homeobox protein SIX1 (SIX1) CSB-YP021348LNL
CSB-EP021348LNL
CSB-BP021348LNL
CSB-MP021348LNL
CSB-EP021348LNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glandirana rugosa LIM/homeobox protein Lhx9 (lhx9) CSB-YP012926GEL
CSB-EP012926GEL
CSB-BP012926GEL
CSB-MP012926GEL
CSB-EP012926GEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solea senegalensis 40S ribosomal protein SA (rpsa) CSB-YP020485STX
CSB-EP020485STX
CSB-BP020485STX
CSB-MP020485STX
CSB-EP020485STX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Mediator of RNA polymerase II transcription subunit 14 (rgr1), partial CSB-YP013647AVE
CSB-EP013647AVE
CSB-BP013647AVE
CSB-MP013647AVE
CSB-EP013647AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Conserved oligomeric Golgi complex subunit 6 (cog6), partial CSB-YP005710AVE
CSB-EP005710AVE
CSB-BP005710AVE
CSB-MP005710AVE
CSB-EP005710AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Eukaryotic translation initiation factor 3 subunit H (An08g01790) CSB-YP007537AVE
CSB-EP007537AVE
CSB-BP007537AVE
CSB-MP007537AVE
CSB-EP007537AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger NADPH--cytochrome P450 reductase (cprA), partial CSB-YP018378AVE1
CSB-EP018378AVE1
CSB-BP018378AVE1
CSB-MP018378AVE1
CSB-EP018378AVE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger DNA mismatch repair protein msh3 (msh3), partial CSB-YP015029AVE
CSB-EP015029AVE
CSB-BP015029AVE
CSB-MP015029AVE
CSB-EP015029AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Cytoplasmic tRNA 2-thiolation protein 2 (ncs2) CSB-YP003313AVE
CSB-EP003313AVE
CSB-BP003313AVE
CSB-MP003313AVE
CSB-EP003313AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Myosin-1 (myoA), partial CSB-YP015289AVE
CSB-EP015289AVE
CSB-BP015289AVE
CSB-MP015289AVE
CSB-EP015289AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Eukaryotic translation initiation factor 3 subunit K (An16g02940) CSB-YP007540AVE
CSB-EP007540AVE
CSB-BP007540AVE
CSB-MP007540AVE
CSB-EP007540AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Eukaryotic translation initiation factor 3 subunit L (An16g04580) CSB-YP007541AVE
CSB-EP007541AVE
CSB-BP007541AVE
CSB-MP007541AVE
CSB-EP007541AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable lysosomal cobalamin transporter (An16g09150), partial CSB-YP012997AVE
CSB-EP012997AVE
CSB-BP012997AVE
CSB-MP012997AVE
CSB-EP012997AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Peptide chain release factor 1 (prfA) CSB-YP015197SUQ
CSB-EP015197SUQ
CSB-BP015197SUQ
CSB-MP015197SUQ
CSB-EP015197SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 tRNA dimethylallyltransferase (miaA) CSB-YP024529SUQ
CSB-EP024529SUQ
CSB-BP024529SUQ
CSB-MP024529SUQ
CSB-EP024529SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 ATP synthase subunit b (atpF), partial CSB-YP002358SUQ1
CSB-EP002358SUQ1
CSB-BP002358SUQ1
CSB-MP002358SUQ1
CSB-EP002358SUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 ATP synthase subunit a (atpB), partial CSB-YP015070SUQ1
CSB-EP015070SUQ1
CSB-BP015070SUQ1
CSB-MP015070SUQ1
CSB-EP015070SUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 GTPase Era (era) CSB-YP007759SUQ
CSB-EP007759SUQ
CSB-BP007759SUQ
CSB-MP007759SUQ
CSB-EP007759SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>