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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Plutella xylostella 60S ribosomal protein L38 (RpL38) CSB-YP738955EXE
CSB-EP738955EXE
CSB-BP738955EXE
CSB-MP738955EXE
CSB-EP738955EXE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Geranylgeranyl pyrophosphate synthase (Ggps1) CSB-YP738956RA
CSB-EP738956RA
CSB-BP738956RA
CSB-MP738956RA
CSB-EP738956RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L34 (rpmH) CSB-YP738957AWW
CSB-EP738957AWW
CSB-BP738957AWW
CSB-MP738957AWW
CSB-EP738957AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Protein grpE (grpE) CSB-YP738958AWW
CSB-EP738958AWW
CSB-BP738958AWW
CSB-MP738958AWW
CSB-EP738958AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP738959AWW
CSB-EP738959AWW
CSB-BP738959AWW
CSB-MP738959AWW
CSB-EP738959AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Probable allantoicase (alc) CSB-YP738960AWW
CSB-EP738960AWW
CSB-BP738960AWW
CSB-MP738960AWW
CSB-EP738960AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP738961AWW
CSB-EP738961AWW
CSB-BP738961AWW
CSB-MP738961AWW
CSB-EP738961AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 3-dehydroquinate synthase (aroB) CSB-YP738962AWW
CSB-EP738962AWW
CSB-BP738962AWW
CSB-MP738962AWW
CSB-EP738962AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. GTP cyclohydrolase-2 (ribA) CSB-YP738963AWW
CSB-EP738963AWW
CSB-BP738963AWW
CSB-MP738963AWW
CSB-EP738963AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 30S ribosomal protein S19 (rpsS) CSB-YP738964AWW
CSB-EP738964AWW
CSB-BP738964AWW
CSB-MP738964AWW
CSB-EP738964AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 30S ribosomal protein S8 (rpsH) CSB-YP738965AWW
CSB-EP738965AWW
CSB-BP738965AWW
CSB-MP738965AWW
CSB-EP738965AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L15 (rplO) CSB-YP738966AWW
CSB-EP738966AWW
CSB-BP738966AWW
CSB-MP738966AWW
CSB-EP738966AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UPF0350 protein ACIAD3189 (ACIAD3189) CSB-YP738967AWW
CSB-EP738967AWW
CSB-BP738967AWW
CSB-MP738967AWW
CSB-EP738967AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP738968AWW1
CSB-EP738968AWW1
CSB-BP738968AWW1
CSB-MP738968AWW1
CSB-EP738968AWW1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L20 (rplT) CSB-YP738969AWW
CSB-EP738969AWW
CSB-BP738969AWW
CSB-MP738969AWW
CSB-EP738969AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. tRNA (Ile)-lysidine synthase (tilS) CSB-YP738970AWW
CSB-EP738970AWW
CSB-BP738970AWW
CSB-MP738970AWW
CSB-EP738970AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Siroheme synthase (cysG) CSB-YP738971AWW
CSB-EP738971AWW
CSB-BP738971AWW
CSB-MP738971AWW
CSB-EP738971AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L25 (rplY) CSB-YP738972AWW
CSB-EP738972AWW
CSB-BP738972AWW
CSB-MP738972AWW
CSB-EP738972AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Protease HtpX (htpX), partial CSB-YP738973AWW1
CSB-EP738973AWW1
CSB-BP738973AWW1
CSB-MP738973AWW1
CSB-EP738973AWW1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP738974AWW
CSB-EP738974AWW
CSB-BP738974AWW
CSB-MP738974AWW
CSB-EP738974AWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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