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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Alkaline phosphatase (phoK), partial CSB-YP001633SUE
CSB-EP001633SUE
CSB-BP001633SUE
CSB-MP001633SUE
CSB-EP001633SUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Kynurenine 3-monooxygenase (cn), partial CSB-YP012475DLU1
CSB-EP012475DLU1
CSB-BP012475DLU1
CSB-MP012475DLU1
CSB-EP012475DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Mediator of RNA polymerase II transcription subunit 8 (MED8) CSB-YP013668DLU
CSB-EP013668DLU
CSB-BP013668DLU
CSB-MP013668DLU
CSB-EP013668DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse REM2- and Rab-like small GTPase 1 (Rsg1) CSB-YP003626MO
CSB-EP003626MO
CSB-BP003626MO
CSB-MP003626MO
CSB-EP003626MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein SZT2 (Szt2), partial CSB-YP012193MO
CSB-EP012193MO
CSB-BP012193MO
CSB-MP012193MO
CSB-EP012193MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse PR domain-containing protein 11 (Prdm11) CSB-YP018640MO
CSB-EP018640MO
CSB-BP018640MO
CSB-MP018640MO
CSB-EP018640MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1 (Ppip5k1), partial CSB-YP010374MO
CSB-EP010374MO
CSB-BP010374MO
CSB-MP010374MO
CSB-EP010374MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Exocyst complex component 3-like protein (exoc3l1), partial CSB-YP007880DIL
CSB-EP007880DIL
CSB-BP007880DIL
CSB-MP007880DIL
CSB-EP007880DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio HORMA domain-containing protein 1 (hormad1) CSB-YP010644DIL
CSB-EP010644DIL
CSB-BP010644DIL
CSB-MP010644DIL
CSB-EP010644DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Calcium-binding and coiled-coil domain-containing protein 1 (calcoco1), partial CSB-YP004436DIL
CSB-EP004436DIL
CSB-BP004436DIL
CSB-MP004436DIL
CSB-EP004436DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Neuronal-specific septin-3 (sept3) CSB-YP021026DIL
CSB-EP021026DIL
CSB-BP021026DIL
CSB-MP021026DIL
CSB-EP021026DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio CCR4-NOT transcription complex subunit 6-like (cnot6l), partial CSB-YP005670DIL
CSB-EP005670DIL
CSB-BP005670DIL
CSB-MP005670DIL
CSB-EP005670DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transmembrane anterior posterior transformation protein 1 homolog (tapt1), partial CSB-YP023124DIL1
CSB-EP023124DIL1
CSB-BP023124DIL1
CSB-MP023124DIL1
CSB-EP023124DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Nucleoside diphosphate kinase (ndk) CSB-YP015889HSR
CSB-EP015889HSR
CSB-BP015889HSR
CSB-MP015889HSR
CSB-EP015889HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824HSR
CSB-EP005824HSR
CSB-BP005824HSR
CSB-MP005824HSR
CSB-EP005824HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Translation initiation factor IF-2 (infB), partial CSB-YP015164PZB
CSB-EP015164PZB
CSB-BP015164PZB
CSB-MP015164PZB
CSB-EP015164PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acyl carrier protein (acpP) CSB-YP015636PZB
CSB-EP015636PZB
CSB-BP015636PZB
CSB-MP015636PZB
CSB-EP015636PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Nucleoside diphosphate kinase (ndk) CSB-YP015889PZG
CSB-EP015889PZG
CSB-BP015889PZG
CSB-MP015889PZG
CSB-EP015889PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146PZG
CSB-EP019146PZG
CSB-BP019146PZG
CSB-MP019146PZG
CSB-EP019146PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150PZG
CSB-EP015150PZG
CSB-BP015150PZG
CSB-MP015150PZG
CSB-EP015150PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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