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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pyogenes serotype M5 Ribosome-recycling factor (frr) CSB-YP014941SUQ
CSB-EP014941SUQ
CSB-BP014941SUQ
CSB-MP014941SUQ
CSB-EP014941SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Elongation factor Ts (tsf) CSB-YP025124SUQ
CSB-EP025124SUQ
CSB-BP025124SUQ
CSB-MP025124SUQ
CSB-EP025124SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Aminopeptidase N (pepN), partial CSB-YP001827LND
CSB-EP001827LND
CSB-BP001827LND
CSB-MP001827LND
CSB-EP001827LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Thymidylate kinase (tmk) CSB-YP007226LND
CSB-EP007226LND
CSB-BP007226LND
CSB-MP007226LND
CSB-EP007226LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Phosphoserine aminotransferase (serC) CSB-YP018838LND
CSB-EP018838LND
CSB-BP018838LND
CSB-MP018838LND
CSB-EP018838LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris tRNA dimethylallyltransferase (miaA) CSB-YP024529LND
CSB-EP024529LND
CSB-BP024529LND
CSB-MP024529LND
CSB-EP024529LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Triosephosphate isomerase (tpiA) CSB-YP024102LND
CSB-EP024102LND
CSB-BP024102LND
CSB-MP024102LND
CSB-EP024102LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Zinc finger and SCAN domain-containing protein 12 (ZSCAN12), partial CSB-YP027132GGZ
CSB-EP027132GGZ
CSB-BP027132GGZ
CSB-MP027132GGZ
CSB-EP027132GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Oxidoreductase HTATIP2 (HTATIP2) CSB-YP010871GGZ
CSB-EP010871GGZ
CSB-BP010871GGZ
CSB-MP010871GGZ
CSB-EP010871GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Retina and anterior neural fold homeobox protein 2 (RAX2) CSB-YP019385GGZ
CSB-EP019385GGZ
CSB-BP019385GGZ
CSB-MP019385GGZ
CSB-EP019385GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Homeobox protein Hox-B7 (HOXB7) CSB-YP010667GGZ
CSB-EP010667GGZ
CSB-BP010667GGZ
CSB-MP010667GGZ
CSB-EP010667GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saguinus labiatus Homeobox protein goosecoid (GSC) CSB-YP009950SVY
CSB-EP009950SVY
CSB-BP009950SVY
CSB-MP009950SVY
CSB-EP009950SVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus E3 ubiquitin-protein ligase RING1 (RING1) CSB-YP019729EQU
CSB-EP019729EQU
CSB-BP019729EQU
CSB-MP019729EQU
CSB-EP019729EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Zinc finger and SCAN domain-containing protein 21 (ZSCAN21) CSB-YP027137EQU
CSB-EP027137EQU
CSB-BP027137EQU
CSB-MP027137EQU
CSB-EP027137EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Sodium-coupled neutral amino acid transporter 2 (SLC38A2), partial CSB-YP021617EQU
CSB-EP021617EQU
CSB-BP021617EQU
CSB-MP021617EQU
CSB-EP021617EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Homeobox protein goosecoid (GSC) CSB-YP009950EQU
CSB-EP009950EQU
CSB-BP009950EQU
CSB-MP009950EQU
CSB-EP009950EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Putative homeobox protein NANOG2 (NANOGP1) CSB-YP015425EQU
CSB-EP015425EQU
CSB-BP015425EQU
CSB-MP015425EQU
CSB-EP015425EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Homeobox protein MOX-1 (MEOX1) CSB-YP013695EQU
CSB-EP013695EQU
CSB-BP013695EQU
CSB-MP013695EQU
CSB-EP013695EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Homeobox protein Hox-A7 (HOXA7) CSB-YP010657EQU
CSB-EP010657EQU
CSB-BP010657EQU
CSB-MP010657EQU
CSB-EP010657EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca nemestrina Agouti-signaling protein (ASIP) CSB-YP002212MOX
CSB-EP002212MOX
CSB-BP002212MOX
CSB-MP002212MOX
CSB-EP002212MOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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