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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter sp. Porphobilinogen deaminase (hemC) CSB-YP739275AWW
CSB-EP739275AWW
CSB-BP739275AWW
CSB-MP739275AWW
CSB-EP739275AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Lipid II flippase FtsW (ftsW), partial CSB-YP739276AWW
CSB-EP739276AWW
CSB-BP739276AWW
CSB-MP739276AWW
CSB-EP739276AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. ATP synthase gamma chain (atpG) CSB-YP739277AWW
CSB-EP739277AWW
CSB-BP739277AWW
CSB-MP739277AWW
CSB-EP739277AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Zinc import ATP-binding protein ZnuC (znuC) CSB-YP739278AWW
CSB-EP739278AWW
CSB-BP739278AWW
CSB-MP739278AWW
CSB-EP739278AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Ribonuclease PH (rph) CSB-YP739279AWW
CSB-EP739279AWW
CSB-BP739279AWW
CSB-MP739279AWW
CSB-EP739279AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (rutB) CSB-YP739280AWW
CSB-EP739280AWW
CSB-BP739280AWW
CSB-MP739280AWW
CSB-EP739280AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP739281AWW
CSB-EP739281AWW
CSB-BP739281AWW
CSB-MP739281AWW
CSB-EP739281AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ceramide synthase subunit LIP1 (LIP1), partial CSB-YP739282CZI
CSB-EP739282CZI
CSB-BP739282CZI
CSB-MP739282CZI
CSB-EP739282CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitogen-activated protein kinase HOG1 (HOG1) CSB-YP739283CZI
CSB-EP739283CZI
CSB-BP739283CZI
CSB-MP739283CZI
CSB-EP739283CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Transcription factor BYE1 (BYE1), partial CSB-YP739284CZI
CSB-EP739284CZI
CSB-BP739284CZI
CSB-MP739284CZI
CSB-EP739284CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Altered inheritance of mitochondria protein 24, mitochondrial (AIM24) CSB-YP739285CZI
CSB-EP739285CZI
CSB-BP739285CZI
CSB-MP739285CZI
CSB-EP739285CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata RNA polymerase II holoenzyme cyclin-like subunit (SSN8) CSB-YP739286CZI
CSB-EP739286CZI
CSB-BP739286CZI
CSB-MP739286CZI
CSB-EP739286CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Oleate activated transcription factor 3 (OAF3), partial CSB-YP739287CZI
CSB-EP739287CZI
CSB-BP739287CZI
CSB-MP739287CZI
CSB-EP739287CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata F-box protein COS111 (COS111), partial CSB-YP739288CZI
CSB-EP739288CZI
CSB-BP739288CZI
CSB-MP739288CZI
CSB-EP739288CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Structure-specific endonuclease subunit SLX4 (SLX4), partial CSB-YP739289CZI
CSB-EP739289CZI
CSB-BP739289CZI
CSB-MP739289CZI
CSB-EP739289CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Chromatin modification-related protein EAF5 (EAF5) CSB-YP739290CZI
CSB-EP739290CZI
CSB-BP739290CZI
CSB-MP739290CZI
CSB-EP739290CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Exocyst complex protein EXO70 (EXO70), partial CSB-YP739291CZI
CSB-EP739291CZI
CSB-BP739291CZI
CSB-MP739291CZI
CSB-EP739291CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Iron sulfur cluster assembly protein 1, mitochondrial (ISU1) CSB-YP739292CZI
CSB-EP739292CZI
CSB-BP739292CZI
CSB-MP739292CZI
CSB-EP739292CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Autophagy-related protein 29 (ATG29) CSB-YP739293CZI
CSB-EP739293CZI
CSB-BP739293CZI
CSB-MP739293CZI
CSB-EP739293CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata FK506-binding protein 4 (FPR4) CSB-YP739294CZI
CSB-EP739294CZI
CSB-BP739294CZI
CSB-MP739294CZI
CSB-EP739294CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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