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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. indica Autophagy protein 5 (ATG5) CSB-YP002293OFF
CSB-EP002293OFF
CSB-BP002293OFF
CSB-MP002293OFF
CSB-EP002293OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Ubiquitin carboxyl-terminal hydrolase 26 (UBP26) CSB-YP025716OFF
CSB-EP025716OFF
CSB-BP025716OFF
CSB-MP025716OFF
CSB-EP025716OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica U1 small nuclear ribonucleoprotein A (OsI_18512) CSB-YP022327OFF
CSB-EP022327OFF
CSB-BP022327OFF
CSB-MP022327OFF
CSB-EP022327OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Serine/threonine-protein kinase ATR (OsI_023634), partial CSB-YP002433OFF
CSB-EP002433OFF
CSB-BP002433OFF
CSB-MP002433OFF
CSB-EP002433OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Pre-mRNA-splicing factor SLU7 (OsI_27673), partial CSB-YP021783OFF
CSB-EP021783OFF
CSB-BP021783OFF
CSB-MP021783OFF
CSB-EP021783OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 40S ribosomal protein S16 (RPS16A) CSB-YP020379OFF
CSB-EP020379OFF
CSB-BP020379OFF
CSB-MP020379OFF
CSB-EP020379OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Methylthioribose-1-phosphate isomerase (OsI_35550) CSB-YP014799OFF
CSB-EP014799OFF
CSB-BP014799OFF
CSB-MP014799OFF
CSB-EP014799OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Serine/threonine-protein kinase Nek2 (NEK2), partial CSB-YP015699OFF
CSB-EP015699OFF
CSB-BP015699OFF
CSB-MP015699OFF
CSB-EP015699OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Actin-related protein 5 (ARP5), partial CSB-YP001252OFG
CSB-EP001252OFG
CSB-BP001252OFG
CSB-MP001252OFG
CSB-EP001252OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SUR
CSB-EP019146SUR
CSB-BP019146SUR
CSB-MP019146SUR
CSB-EP019146SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis ATP synthase subunit delta (atpH) CSB-YP002355SUR
CSB-EP002355SUR
CSB-BP002355SUR
CSB-MP002355SUR
CSB-EP002355SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Peptide chain release factor 1 (prfA) CSB-YP015197SUR
CSB-EP015197SUR
CSB-BP015197SUR
CSB-MP015197SUR
CSB-EP015197SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Ribonuclease 3 (rnc) CSB-YP019809SUR
CSB-EP019809SUR
CSB-BP019809SUR
CSB-MP019809SUR
CSB-EP019809SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SUR
CSB-EP015150SUR
CSB-BP015150SUR
CSB-MP015150SUR
CSB-EP015150SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Translation initiation factor IF-2 (infB), partial CSB-YP015164SUR
CSB-EP015164SUR
CSB-BP015164SUR
CSB-MP015164SUR
CSB-EP015164SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Transcription initiation factor IIB (tfb) CSB-YP010001MNU
CSB-EP010001MNU
CSB-BP010001MNU
CSB-MP010001MNU
CSB-EP010001MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Protein pelota homolog (pelA) CSB-YP017778MNU
CSB-EP017778MNU
CSB-BP017778MNU
CSB-MP017778MNU
CSB-EP017778MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Triosephosphate isomerase (tpiA) CSB-YP024102MNU
CSB-EP024102MNU
CSB-BP024102MNU
CSB-MP024102MNU
CSB-EP024102MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri tRNA pseudouridine synthase A (truA) CSB-YP019086MNU
CSB-EP019086MNU
CSB-BP019086MNU
CSB-MP019086MNU
CSB-EP019086MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033STL
CSB-EP018033STL
CSB-BP018033STL
CSB-MP018033STL
CSB-EP018033STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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