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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Glycine cleavage system H protein (gcvH) CSB-YP009335STL
CSB-EP009335STL
CSB-BP009335STL
CSB-MP009335STL
CSB-EP009335STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica NAD-dependent malic enzyme (maeA), partial CSB-YP013633STL
CSB-EP013633STL
CSB-BP013633STL
CSB-MP013633STL
CSB-EP013633STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Transaldolase (tal) CSB-YP023112STL
CSB-EP023112STL
CSB-BP023112STL
CSB-MP023112STL
CSB-EP023112STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Cysteine desulfurase (iscS) CSB-YP015769STL
CSB-EP015769STL
CSB-BP015769STL
CSB-MP015769STL
CSB-EP015769STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917STL
CSB-EP022917STL
CSB-BP022917STL
CSB-MP022917STL
CSB-EP022917STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 3-ketoacyl-CoA thiolase (fadI), partial CSB-YP001117STL
CSB-EP001117STL
CSB-BP001117STL
CSB-MP001117STL
CSB-EP001117STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582STL
CSB-EP009582STL
CSB-BP009582STL
CSB-MP009582STL
CSB-EP009582STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Urocanate hydratase (hutU), partial CSB-YP025677STL
CSB-EP025677STL
CSB-BP025677STL
CSB-MP025677STL
CSB-EP025677STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP synthase subunit beta (atpD) CSB-YP002350STL
CSB-EP002350STL
CSB-BP002350STL
CSB-MP002350STL
CSB-EP002350STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP synthase subunit delta (atpH) CSB-YP002355STL
CSB-EP002355STL
CSB-BP002355STL
CSB-MP002355STL
CSB-EP002355STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Triosephosphate isomerase (tpiA) CSB-YP024102DUT
CSB-EP024102DUT
CSB-BP024102DUT
CSB-MP024102DUT
CSB-EP024102DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Nucleoside diphosphate kinase (ndk) CSB-YP015889DUT
CSB-EP015889DUT
CSB-BP015889DUT
CSB-MP015889DUT
CSB-EP015889DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum tRNA dimethylallyltransferase (miaA) CSB-YP024529DUT
CSB-EP024529DUT
CSB-BP024529DUT
CSB-MP024529DUT
CSB-EP024529DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Acyl carrier protein (acpP) CSB-YP015636DUT
CSB-EP015636DUT
CSB-BP015636DUT
CSB-MP015636DUT
CSB-EP015636DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Elongation factor Ts (tsf) CSB-YP025124DUT
CSB-EP025124DUT
CSB-BP025124DUT
CSB-MP025124DUT
CSB-EP025124DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Serine hydroxymethyltransferase (glyA) CSB-YP021274DUT
CSB-EP021274DUT
CSB-BP021274DUT
CSB-MP021274DUT
CSB-EP021274DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Peptide chain release factor 1 (prfA) CSB-YP015197DUT
CSB-EP015197DUT
CSB-BP015197DUT
CSB-MP015197DUT
CSB-EP015197DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP synthase subunit b (atpF), partial CSB-YP002358DUT1
CSB-EP002358DUT1
CSB-BP002358DUT1
CSB-MP002358DUT1
CSB-EP002358DUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP synthase subunit beta (atpD) CSB-YP002350DUT
CSB-EP002350DUT
CSB-BP002350DUT
CSB-MP002350DUT
CSB-EP002350DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Elongation factor G (fusA), partial CSB-YP009373DUT
CSB-EP009373DUT
CSB-BP009373DUT
CSB-MP009373DUT
CSB-EP009373DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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