Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Probable ATP-dependent RNA helicase DHX40 (Dhx40) CSB-YP740775MO
CSB-EP740775MO
CSB-BP740775MO
CSB-MP740775MO
CSB-EP740775MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tubulin-specific chaperone A (Tbca) CSB-YP740776RA
CSB-EP740776RA
CSB-BP740776RA
CSB-MP740776RA
CSB-EP740776RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse WAS/WASL-interacting protein family member 2 (Wipf2) CSB-YP740777MO
CSB-EP740777MO
CSB-BP740777MO
CSB-MP740777MO
CSB-EP740777MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Podocan-like protein 1 (PODNL1) CSB-YP740778HU
CSB-EP740778HU
CSB-BP740778HU
CSB-MP740778HU
CSB-EP740778HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Actin filament-associated protein 1-like 2 (afap1l2), partial CSB-YP740779XBE
CSB-EP740779XBE
CSB-BP740779XBE
CSB-MP740779XBE
CSB-EP740779XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nuclear apoptosis-inducing factor 1 (Naif1) CSB-YP740780MO
CSB-EP740780MO
CSB-BP740780MO
CSB-MP740780MO
CSB-EP740780MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio ER lumen protein retaining receptor 3 (kdelr3), partial CSB-YP740781DIL1
CSB-EP740781DIL1
CSB-BP740781DIL1
CSB-MP740781DIL1
CSB-EP740781DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Dual specificity protein phosphatase CDC14B (Cdc14b) CSB-YP740782MO
CSB-EP740782MO
CSB-BP740782MO
CSB-MP740782MO
CSB-EP740782MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase DCLK2 (Dclk2) CSB-YP740783MO
CSB-EP740783MO
CSB-BP740783MO
CSB-MP740783MO
CSB-EP740783MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Leucine-rich repeat and fibronectin type III domain-containing protein 1 (lrfn1), partial CSB-YP740784DIL
CSB-EP740784DIL
CSB-BP740784DIL
CSB-MP740784DIL
CSB-EP740784DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Solute carrier family 25 member 34 (slc25a34), partial CSB-YP740785DIL1
CSB-EP740785DIL1
CSB-BP740785DIL1
CSB-MP740785DIL1
CSB-EP740785DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Programmed cell death protein 10-A (pdcd10a) CSB-YP740786DIL
CSB-EP740786DIL
CSB-BP740786DIL
CSB-MP740786DIL
CSB-EP740786DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sortilin (Sort1), partial CSB-YP740787MO
CSB-EP740787MO
CSB-BP740787MO
CSB-MP740787MO
CSB-EP740787MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Histone H3.3 (h3f3a) CSB-YP740788DIL
CSB-EP740788DIL
CSB-BP740788DIL
CSB-MP740788DIL
CSB-EP740788DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Unconventional myosin-VIIb (MYO7B), partial CSB-YP740789HU
CSB-EP740789HU
CSB-BP740789HU
CSB-MP740789HU
CSB-EP740789HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Hydroxyacylglutathione hydrolase-like protein (HAGHL) CSB-YP740790HU
CSB-EP740790HU
CSB-BP740790HU
CSB-MP740790HU
CSB-EP740790HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neutral cholesterol ester hydrolase 1 (NCEH1), partial CSB-YP740791HU
CSB-BP740791HU
CSB-MP740791HU
CSB-EP740791HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C14orf43 (C14orf43), partial CSB-YP740792HU
CSB-EP740792HU
CSB-BP740792HU
CSB-MP740792HU
CSB-EP740792HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger CCCH domain-containing protein 14 (ZC3H14) CSB-EP740793HU
CSB-BP740793HU
CSB-MP740793HU
CSB-EP740793HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cancer/testis antigen 75 (CT75) CSB-YP740794HU
CSB-EP740794HU
CSB-BP740794HU
CSB-MP740794HU
CSB-EP740794HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>