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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP741355BQN
CSB-EP741355BQN
CSB-BP741355BQN
CSB-MP741355BQN
CSB-EP741355BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Guanylate kinase (gmk) CSB-YP741356BQN
CSB-EP741356BQN
CSB-BP741356BQN
CSB-MP741356BQN
CSB-EP741356BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Transcription factor fapR (fapR) CSB-YP741357BQN
CSB-EP741357BQN
CSB-BP741357BQN
CSB-MP741357BQN
CSB-EP741357BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP741358BQN
CSB-EP741358BQN
CSB-BP741358BQN
CSB-MP741358BQN
CSB-EP741358BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosome-binding factor A (rbfA) CSB-YP741359BQN
CSB-EP741359BQN
CSB-BP741359BQN
CSB-MP741359BQN
CSB-EP741359BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB), partial CSB-YP741360BQN
CSB-EP741360BQN
CSB-BP741360BQN
CSB-MP741360BQN
CSB-EP741360BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0154 protein BCE_3716 (BCE_3716), partial CSB-YP741361BQN
CSB-EP741361BQN
CSB-BP741361BQN
CSB-MP741361BQN
CSB-EP741361BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0337 protein BCE_3655 (BCE_3655) CSB-YP741362BQN
CSB-EP741362BQN
CSB-BP741362BQN
CSB-MP741362BQN
CSB-EP741362BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoserine aminotransferase (serC) CSB-YP741363BQN
CSB-EP741363BQN
CSB-BP741363BQN
CSB-MP741363BQN
CSB-EP741363BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0271 protein BCE_3119 (BCE_3119) CSB-YP741364BQN
CSB-EP741364BQN
CSB-BP741364BQN
CSB-MP741364BQN
CSB-EP741364BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Kynureninase (kynU) CSB-YP741365BQN
CSB-EP741365BQN
CSB-BP741365BQN
CSB-MP741365BQN
CSB-EP741365BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylmalonate semialdehyde dehydrogenase [acylating] (iolA) CSB-YP741366BQN
CSB-EP741366BQN
CSB-BP741366BQN
CSB-MP741366BQN
CSB-EP741366BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Xylose isomerase (xylA) CSB-YP741367BQN
CSB-EP741367BQN
CSB-BP741367BQN
CSB-MP741367BQN
CSB-EP741367BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-hydroxy-2-oxovalerate aldolase (BCE_2157) CSB-YP741368BQN
CSB-EP741368BQN
CSB-BP741368BQN
CSB-MP741368BQN
CSB-EP741368BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADPH dehydrogenase (namA) CSB-YP741369BQN
CSB-EP741369BQN
CSB-BP741369BQN
CSB-MP741369BQN
CSB-EP741369BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Xanthine phosphoribosyltransferase (xpt) CSB-YP741370BQN
CSB-EP741370BQN
CSB-BP741370BQN
CSB-MP741370BQN
CSB-EP741370BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylglyoxal synthase (mgsA) CSB-YP741371BQN
CSB-EP741371BQN
CSB-BP741371BQN
CSB-MP741371BQN
CSB-EP741371BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP741372BQN
CSB-EP741372BQN
CSB-BP741372BQN
CSB-MP741372BQN
CSB-EP741372BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-isopropylmalate dehydrogenase (leuB) CSB-YP741373BQN
CSB-EP741373BQN
CSB-BP741373BQN
CSB-MP741373BQN
CSB-EP741373BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lactate utilization protein C (lutC) CSB-YP741374BQN
CSB-EP741374BQN
CSB-BP741374BQN
CSB-MP741374BQN
CSB-EP741374BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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