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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bordetella bronchiseptica Beta-hexosaminidase (nagZ) CSB-YP743057BTY
CSB-EP743057BTY
CSB-BP743057BTY
CSB-MP743057BTY
CSB-EP743057BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP743058BTY
CSB-EP743058BTY
CSB-BP743058BTY
CSB-MP743058BTY
CSB-EP743058BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 30S ribosomal protein S16 (rpsP) CSB-YP743059BTY
CSB-EP743059BTY
CSB-BP743059BTY
CSB-MP743059BTY
CSB-EP743059BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP743060BTY
CSB-EP743060BTY
CSB-BP743060BTY
CSB-MP743060BTY
CSB-EP743060BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP743061BTY
CSB-EP743061BTY
CSB-BP743061BTY
CSB-MP743061BTY
CSB-EP743061BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica sn-glycerol-3-phosphate import ATP-binding protein UgpC (ugpC) CSB-YP743062BTY
CSB-EP743062BTY
CSB-BP743062BTY
CSB-MP743062BTY
CSB-EP743062BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Periplasmic nitrate reductase (napA), partial CSB-YP743063BTY
CSB-EP743063BTY
CSB-BP743063BTY
CSB-MP743063BTY
CSB-EP743063BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Lipid-A-disaccharide synthase (lpxB) CSB-YP743064BTY
CSB-EP743064BTY
CSB-BP743064BTY
CSB-MP743064BTY
CSB-EP743064BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica [Protein-PII] uridylyltransferase (glnD), partial CSB-YP743065BTY
CSB-EP743065BTY
CSB-BP743065BTY
CSB-MP743065BTY
CSB-EP743065BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Probable chorismate--pyruvate lyase (ubiC) CSB-YP743066BTY
CSB-EP743066BTY
CSB-BP743066BTY
CSB-MP743066BTY
CSB-EP743066BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Lysine--tRNA ligase (lysS), partial CSB-YP743067BTY
CSB-EP743067BTY
CSB-BP743067BTY
CSB-MP743067BTY
CSB-EP743067BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Isoleucine--tRNA ligase (ileS), partial CSB-YP743068BTY
CSB-EP743068BTY
CSB-BP743068BTY
CSB-MP743068BTY
CSB-EP743068BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 3-isopropylmalate dehydrogenase 2 (leuB2) CSB-YP743069BTY
CSB-EP743069BTY
CSB-BP743069BTY
CSB-MP743069BTY
CSB-EP743069BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Recombination-associated protein RdgC (rdgC) CSB-YP743070BTY
CSB-EP743070BTY
CSB-BP743070BTY
CSB-MP743070BTY
CSB-EP743070BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 50S ribosomal protein L20 (rplT) CSB-YP743071BTY
CSB-EP743071BTY
CSB-BP743071BTY
CSB-MP743071BTY
CSB-EP743071BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 30S ribosomal protein S20 (rpsT) CSB-YP743072BTY
CSB-EP743072BTY
CSB-BP743072BTY
CSB-MP743072BTY
CSB-EP743072BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP743073BTY
CSB-EP743073BTY
CSB-BP743073BTY
CSB-MP743073BTY
CSB-EP743073BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Maf-like protein BB1865 (BB1865) CSB-YP743074BTY
CSB-EP743074BTY
CSB-BP743074BTY
CSB-MP743074BTY
CSB-EP743074BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP743075BTY
CSB-EP743075BTY
CSB-BP743075BTY
CSB-MP743075BTY
CSB-EP743075BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Phosphate import ATP-binding protein PstB (pstB) CSB-YP743076BTY
CSB-EP743076BTY
CSB-BP743076BTY
CSB-MP743076BTY
CSB-EP743076BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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