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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella bronchiseptica 50S ribosomal protein L34 (rpmH) CSB-YP743037BTY
CSB-EP743037BTY
CSB-BP743037BTY
CSB-MP743037BTY
CSB-EP743037BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Protein smg homolog (smg) CSB-YP743038BTY
CSB-EP743038BTY
CSB-BP743038BTY
CSB-MP743038BTY
CSB-EP743038BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Type IV secretion system protein ptlF homolog (ptlF) CSB-YP743039BTY
CSB-EP743039BTY
CSB-BP743039BTY
CSB-MP743039BTY
CSB-EP743039BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Pertussis toxin subunit 1 homolog (ptxA) CSB-YP743040BTY
CSB-BP743040BTY
CSB-MP743040BTY
CSB-EP743040BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP743041BTY
CSB-EP743041BTY
CSB-BP743041BTY
CSB-MP743041BTY
CSB-EP743041BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP743042BTY
CSB-EP743042BTY
CSB-BP743042BTY
CSB-MP743042BTY
CSB-EP743042BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Probable thiol:disulfide interchange protein DsbC (dsbC) CSB-YP743043BTY
CSB-EP743043BTY
CSB-BP743043BTY
CSB-MP743043BTY
CSB-EP743043BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica ATP synthase subunit beta (atpD) CSB-YP743044BTY
CSB-EP743044BTY
CSB-BP743044BTY
CSB-MP743044BTY
CSB-EP743044BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP743045BTY
CSB-EP743045BTY
CSB-BP743045BTY
CSB-MP743045BTY
CSB-EP743045BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP743046BTY
CSB-EP743046BTY
CSB-BP743046BTY
CSB-MP743046BTY
CSB-EP743046BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Putative Holliday junction resolvase (BB4404) CSB-YP743047BTY
CSB-EP743047BTY
CSB-BP743047BTY
CSB-MP743047BTY
CSB-EP743047BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 30S ribosomal protein S9 (rpsI) CSB-YP743048BTY
CSB-EP743048BTY
CSB-BP743048BTY
CSB-MP743048BTY
CSB-EP743048BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Urease accessory protein UreF (ureF) CSB-YP743049BTY
CSB-EP743049BTY
CSB-BP743049BTY
CSB-MP743049BTY
CSB-EP743049BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Protein MraZ (mraZ) CSB-YP743050BTY
CSB-EP743050BTY
CSB-BP743050BTY
CSB-MP743050BTY
CSB-EP743050BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0324 membrane protein BB4178 (BB4178), partial CSB-YP743051BTY
CSB-EP743051BTY
CSB-BP743051BTY
CSB-MP743051BTY
CSB-EP743051BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Fumarate hydratase class II (fumC) CSB-YP743052BTY
CSB-EP743052BTY
CSB-BP743052BTY
CSB-MP743052BTY
CSB-EP743052BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Putative arginyl-tRNA--protein transferase (ate) CSB-YP743053BTY
CSB-EP743053BTY
CSB-BP743053BTY
CSB-MP743053BTY
CSB-EP743053BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Heat-inducible transcription repressor HrcA (hrcA) CSB-YP743054BTY
CSB-EP743054BTY
CSB-BP743054BTY
CSB-MP743054BTY
CSB-EP743054BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP743055BTY
CSB-EP743055BTY
CSB-BP743055BTY
CSB-MP743055BTY
CSB-EP743055BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP743056BTY1
CSB-EP743056BTY1
CSB-BP743056BTY1
CSB-MP743056BTY1
CSB-EP743056BTY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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