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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP744678LEV
CSB-EP744678LEV
CSB-BP744678LEV
CSB-MP744678LEV
CSB-EP744678LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Phosphoribosylamine--glycine ligase (purD) CSB-YP744679LEV
CSB-EP744679LEV
CSB-BP744679LEV
CSB-MP744679LEV
CSB-EP744679LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni N utilization substance protein B homolog (nusB) CSB-YP744680LEV
CSB-EP744680LEV
CSB-BP744680LEV
CSB-MP744680LEV
CSB-EP744680LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 7-cyano-7-deazaguanine synthase (queC) CSB-YP744681LEV
CSB-EP744681LEV
CSB-BP744681LEV
CSB-MP744681LEV
CSB-EP744681LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni tRNA modification GTPase MnmE (mnmE) CSB-YP744682LEV
CSB-EP744682LEV
CSB-BP744682LEV
CSB-MP744682LEV
CSB-EP744682LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Probable GTP-binding protein EngB (engB) CSB-YP744683LEV
CSB-EP744683LEV
CSB-BP744683LEV
CSB-MP744683LEV
CSB-EP744683LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Uncharacterized RNA methyltransferase LIC_10086 (LIC_10086) CSB-YP744684LEV
CSB-EP744684LEV
CSB-BP744684LEV
CSB-MP744684LEV
CSB-EP744684LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris CRISPR-associated endoribonuclease Cas2 (cas2) CSB-YP744685DDH
CSB-EP744685DDH
CSB-BP744685DDH
CSB-MP744685DDH
CSB-EP744685DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Nucleoid occlusion protein (noc) CSB-YP744686BQN
CSB-EP744686BQN
CSB-BP744686BQN
CSB-MP744686BQN
CSB-EP744686BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Adenylosuccinate synthetase (purA) CSB-YP744687BQN
CSB-EP744687BQN
CSB-BP744687BQN
CSB-MP744687BQN
CSB-EP744687BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GMP reductase (guaC) CSB-YP744688BQN
CSB-EP744688BQN
CSB-BP744688BQN
CSB-MP744688BQN
CSB-EP744688BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L31 type B (rpmE2) CSB-YP744689BQN
CSB-EP744689BQN
CSB-BP744689BQN
CSB-MP744689BQN
CSB-EP744689BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0059 membrane protein BCE_5451 (BCE_5451), partial CSB-YP744690BQN1
CSB-EP744690BQN1
CSB-BP744690BQN1
CSB-MP744690BQN1
CSB-EP744690BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP744691BQN
CSB-EP744691BQN
CSB-BP744691BQN
CSB-MP744691BQN
CSB-EP744691BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0145 protein BCE_5284 (BCE_5284) CSB-YP744692BQN
CSB-EP744692BQN
CSB-BP744692BQN
CSB-MP744692BQN
CSB-EP744692BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP744693BQN
CSB-EP744693BQN
CSB-BP744693BQN
CSB-MP744693BQN
CSB-EP744693BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycine cleavage system H protein (gcvH) CSB-YP744694BQN
CSB-EP744694BQN
CSB-BP744694BQN
CSB-MP744694BQN
CSB-EP744694BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ferredoxin--NADP reductase 2 (BCE_5065) CSB-YP744695BQN
CSB-EP744695BQN
CSB-BP744695BQN
CSB-MP744695BQN
CSB-EP744695BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP744696BQN
CSB-EP744696BQN
CSB-BP744696BQN
CSB-MP744696BQN
CSB-EP744696BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 6-phosphofructokinase (pfkA) CSB-YP744697BQN
CSB-EP744697BQN
CSB-BP744697BQN
CSB-MP744697BQN
CSB-EP744697BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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