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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella pertussis Urease accessory protein UreE (ureE) CSB-YP742937BUA
CSB-EP742937BUA
CSB-BP742937BUA
CSB-MP742937BUA
CSB-EP742937BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP742938BUA
CSB-EP742938BUA
CSB-BP742938BUA
CSB-MP742938BUA
CSB-EP742938BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis S-adenosylmethionine synthase (metK) CSB-YP742939BUA
CSB-EP742939BUA
CSB-BP742939BUA
CSB-MP742939BUA
CSB-EP742939BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Cell division protein FtsL (ftsL), partial CSB-YP742940BUA
CSB-EP742940BUA
CSB-BP742940BUA
CSB-MP742940BUA
CSB-EP742940BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP742941BUA
CSB-EP742941BUA
CSB-BP742941BUA
CSB-MP742941BUA
CSB-EP742941BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 30S ribosomal protein S9 (rpsI) CSB-YP742942BUA
CSB-EP742942BUA
CSB-BP742942BUA
CSB-MP742942BUA
CSB-EP742942BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis UPF0234 protein BP2916 (BP2916) CSB-YP742943BUA
CSB-EP742943BUA
CSB-BP742943BUA
CSB-MP742943BUA
CSB-EP742943BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 30S ribosomal protein S18 (rpsR) CSB-YP742944BUA
CSB-EP742944BUA
CSB-BP742944BUA
CSB-MP742944BUA
CSB-EP742944BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Biotin synthase (bioB) CSB-YP742945BUA
CSB-EP742945BUA
CSB-BP742945BUA
CSB-MP742945BUA
CSB-EP742945BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 50S ribosomal protein L35 (rpmI) CSB-YP742946BUA
CSB-EP742946BUA
CSB-BP742946BUA
CSB-MP742946BUA
CSB-EP742946BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Porphobilinogen deaminase (hemC) CSB-YP742947BUA
CSB-EP742947BUA
CSB-BP742947BUA
CSB-MP742947BUA
CSB-EP742947BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Outer-membrane lipoprotein carrier protein (lolA) CSB-YP742948BUA
CSB-EP742948BUA
CSB-BP742948BUA
CSB-MP742948BUA
CSB-EP742948BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Acyl carrier protein (acpP) CSB-YP742949BUA
CSB-EP742949BUA
CSB-BP742949BUA
CSB-MP742949BUA
CSB-EP742949BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Enolase (eno) CSB-YP742950BUA
CSB-EP742950BUA
CSB-BP742950BUA
CSB-MP742950BUA
CSB-EP742950BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP742951BUA
CSB-EP742951BUA
CSB-BP742951BUA
CSB-MP742951BUA
CSB-EP742951BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis GTPase Der (der) CSB-YP742952BUA
CSB-EP742952BUA
CSB-BP742952BUA
CSB-MP742952BUA
CSB-EP742952BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP742953BUA
CSB-EP742953BUA
CSB-BP742953BUA
CSB-MP742953BUA
CSB-EP742953BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis NAD-dependent protein deacetylase (cobB) CSB-YP742954BUA
CSB-EP742954BUA
CSB-BP742954BUA
CSB-MP742954BUA
CSB-EP742954BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Thymidylate kinase (tmk) CSB-YP742955BUA
CSB-EP742955BUA
CSB-BP742955BUA
CSB-MP742955BUA
CSB-EP742955BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP742956BUA
CSB-EP742956BUA
CSB-BP742956BUA
CSB-MP742956BUA
CSB-EP742956BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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