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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Hippocampus comes 60S ribosomal protein L35 (rpl35) CSB-YP744277HHF
CSB-EP744277HHF
CSB-BP744277HHF
CSB-MP744277HHF
CSB-EP744277HHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Dipeptidyl peptidase 8 (DPP8), partial CSB-YP744278HU
CSB-EP744278HU
CSB-BP744278HU
CSB-MP744278HU
CSB-EP744278HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Dihydroorotate dehydrogenase (quinone), mitochondrial (URA9), partial CSB-YP744279LMA1
CSB-EP744279LMA1
CSB-BP744279LMA1
CSB-MP744279LMA1
CSB-EP744279LMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-ubiquinone oxidoreductase chain 5 (nad5), partial CSB-YP744280ETL
CSB-EP744280ETL
CSB-BP744280ETL
CSB-MP744280ETL
CSB-EP744280ETL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Antileukoproteinase (SLPI) CSB-YP744281SH
CSB-EP744281SH
CSB-BP744281SH
CSB-MP744281SH
CSB-EP744281SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gossypium hirsutum Tubulin alpha-2 chain CSB-YP744282GHB
CSB-EP744282GHB
CSB-BP744282GHB
CSB-MP744282GHB
CSB-EP744282GHB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Forkhead box protein D4-like 2 (FOXD4L2) CSB-YP744283HU
CSB-EP744283HU
CSB-BP744283HU
CSB-MP744283HU
CSB-EP744283HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Heat stress transcription factor A-2b (HSFA2B) CSB-YP744284OFG
CSB-EP744284OFG
CSB-BP744284OFG
CSB-MP744284OFG
CSB-EP744284OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM138A/B/C/F (FAM138A) CSB-YP744285HU
CSB-EP744285HU
CSB-BP744285HU
CSB-MP744285HU
CSB-EP744285HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Aminotransferase ALD1 homolog (ALD1) CSB-YP744286OFG
CSB-EP744286OFG
CSB-BP744286OFG
CSB-MP744286OFG
CSB-EP744286OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Choristoneura fumiferana defective polyhedrosis virus Viral cathepsin (Vcath) CSB-YP744287CHAK
CSB-EP744287CHAK
CSB-BP744287CHAK
CSB-MP744287CHAK
CSB-EP744287CHAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Vitamin D 25-hydroxylase (CYP2R1) CSB-YP744288HU
CSB-EP744288HU
CSB-BP744288HU
CSB-MP744288HU
CSB-EP744288HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Hematopoietic SH2 domain-containing protein (Hsh2d) CSB-YP744289MO
CSB-EP744289MO
CSB-BP744289MO
CSB-MP744289MO
CSB-EP744289MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Developmental pluripotency-associated protein 3 (DPPA3) CSB-YP744290HU
CSB-EP744290HU
CSB-BP744290HU
CSB-MP744290HU
CSB-EP744290HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. 60 kDa chaperonin 2 (groL2), partial CSB-YP744291RKX
CSB-EP744291RKX
CSB-BP744291RKX
CSB-MP744291RKX
CSB-EP744291RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. 7-cyano-7-deazaguanine synthase (queC) CSB-YP744292RKX
CSB-EP744292RKX
CSB-BP744292RKX
CSB-MP744292RKX
CSB-EP744292RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse C-X-C motif chemokine 3 (Cxcl3) CSB-YP744293MO
CSB-EP744293MO
CSB-BP744293MO
CSB-MP744293MO
CSB-EP744293MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Purkinje cell protein 4-like protein 1 (Pcp4l1) CSB-YP744294MO
CSB-EP744294MO
CSB-BP744294MO
CSB-MP744294MO
CSB-EP744294MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB (pstB) CSB-YP744295AGN
CSB-EP744295AGN
CSB-BP744295AGN
CSB-MP744295AGN
CSB-EP744295AGN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Myosin phosphatase Rho-interacting protein (MPRIP), partial CSB-YP744296HU
CSB-EP744296HU
CSB-BP744296HU
CSB-MP744296HU
CSB-EP744296HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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