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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus tropicalis Proteasomal ubiquitin receptor ADRM1 (adrm1) CSB-YP744077XBF
CSB-EP744077XBF
CSB-BP744077XBF
CSB-MP744077XBF
CSB-EP744077XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Lariat debranching enzyme (dbr1), partial CSB-YP744078XBF
CSB-EP744078XBF
CSB-BP744078XBF
CSB-MP744078XBF
CSB-EP744078XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Trimeric intracellular cation channel type A (tmem38a), partial CSB-YP744079XBF1
CSB-EP744079XBF1
CSB-BP744079XBF1
CSB-MP744079XBF1
CSB-EP744079XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (Alg8), partial CSB-YP744080MO
CSB-EP744080MO
CSB-BP744080MO
CSB-MP744080MO
CSB-EP744080MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sorting nexin-6 (Snx6) CSB-YP744081MO
CSB-EP744081MO
CSB-BP744081MO
CSB-MP744081MO
CSB-EP744081MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C15orf26 homolog CSB-YP744082MO
CSB-EP744082MO
CSB-BP744082MO
CSB-MP744082MO
CSB-EP744082MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human GTPase IMAP family member 6 (GIMAP6) CSB-YP744083HU
CSB-EP744083HU
CSB-BP744083HU
CSB-MP744083HU
CSB-EP744083HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse FH1/FH2 domain-containing protein 1 (Fhod1), partial CSB-YP744084MO
CSB-EP744084MO
CSB-BP744084MO
CSB-MP744084MO
CSB-EP744084MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Polypeptide N-acetylgalactosaminyltransferase 10 (Galnt10), partial CSB-YP744085MO
CSB-EP744085MO
CSB-BP744085MO
CSB-MP744085MO
CSB-EP744085MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger protein 82 (Zfp82) CSB-YP744086MO
CSB-EP744086MO
CSB-BP744086MO
CSB-MP744086MO
CSB-EP744086MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Coiled-coil domain-containing protein 22 (ccdc22), partial CSB-YP744087XBE
CSB-EP744087XBE
CSB-BP744087XBE
CSB-MP744087XBE
CSB-EP744087XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Differentially expressed in FDCP 6 homolog (def6), partial CSB-YP744088XBE
CSB-EP744088XBE
CSB-BP744088XBE
CSB-MP744088XBE
CSB-EP744088XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C2orf71 homolog, partial CSB-YP744089MO
CSB-EP744089MO
CSB-BP744089MO
CSB-MP744089MO
CSB-EP744089MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Non-structural maintenance of chromosomes element 1 homolog (nsmce1) CSB-YP744090XBE
CSB-EP744090XBE
CSB-BP744090XBE
CSB-MP744090XBE
CSB-EP744090XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DENN domain-containing protein 5A (Dennd5a), partial CSB-YP744091MO
CSB-EP744091MO
CSB-BP744091MO
CSB-MP744091MO
CSB-EP744091MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-taxilin (Txlna) CSB-YP744092MO
CSB-EP744092MO
CSB-BP744092MO
CSB-MP744092MO
CSB-EP744092MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein CDKN2AIP homolog B (cdkn2aip-b) CSB-YP744093XBE
CSB-EP744093XBE
CSB-BP744093XBE
CSB-MP744093XBE
CSB-EP744093XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Phospholipase D3 (pld3), partial CSB-YP744094XBE
CSB-EP744094XBE
CSB-BP744094XBE
CSB-MP744094XBE
CSB-EP744094XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein BHLHb9 (Bhlhb9) CSB-YP744095MO
CSB-EP744095MO
CSB-BP744095MO
CSB-MP744095MO
CSB-EP744095MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis 60S ribosomal protein L35 (rpl35) CSB-YP744096XBF
CSB-EP744096XBF
CSB-BP744096XBF
CSB-MP744096XBF
CSB-EP744096XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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