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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant RNA pyrophosphohydrolase (rppH) CSB-YP742997BIK
CSB-EP742997BIK
CSB-BP742997BIK
CSB-MP742997BIK
CSB-EP742997BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD), partial CSB-YP742998BIK
CSB-EP742998BIK
CSB-BP742998BIK
CSB-MP742998BIK
CSB-EP742998BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0324 membrane protein BPP3732 (BPP3732), partial CSB-YP742999BIK
CSB-EP742999BIK
CSB-BP742999BIK
CSB-MP742999BIK
CSB-EP742999BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP743000BIK
CSB-EP743000BIK
CSB-BP743000BIK
CSB-MP743000BIK
CSB-EP743000BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP743001BIK
CSB-EP743001BIK
CSB-BP743001BIK
CSB-MP743001BIK
CSB-EP743001BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate reductase (dapB) CSB-YP743002BIK
CSB-EP743002BIK
CSB-BP743002BIK
CSB-MP743002BIK
CSB-EP743002BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP743003BIK
CSB-EP743003BIK
CSB-BP743003BIK
CSB-MP743003BIK
CSB-EP743003BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP743004BIK
CSB-EP743004BIK
CSB-BP743004BIK
CSB-MP743004BIK
CSB-EP743004BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase alpha chain (trpA) CSB-YP743005BIK
CSB-EP743005BIK
CSB-BP743005BIK
CSB-MP743005BIK
CSB-EP743005BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease 3 (rnc) CSB-YP743006BIK
CSB-EP743006BIK
CSB-BP743006BIK
CSB-MP743006BIK
CSB-EP743006BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan--tRNA ligase (trpS) CSB-YP743007BIK
CSB-EP743007BIK
CSB-BP743007BIK
CSB-MP743007BIK
CSB-EP743007BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Integration host factor subunit beta (ihfB) CSB-YP743008BIK
CSB-EP743008BIK
CSB-BP743008BIK
CSB-MP743008BIK
CSB-EP743008BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0317 protein BPP3031 (BPP3031) CSB-YP743009BIK
CSB-EP743009BIK
CSB-BP743009BIK
CSB-MP743009BIK
CSB-EP743009BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S16 (rpsP) CSB-YP743010BIK
CSB-EP743010BIK
CSB-BP743010BIK
CSB-MP743010BIK
CSB-EP743010BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase 1 (hisC1) CSB-YP743011BIK
CSB-EP743011BIK
CSB-BP743011BIK
CSB-MP743011BIK
CSB-EP743011BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP743012BIK
CSB-EP743012BIK
CSB-BP743012BIK
CSB-MP743012BIK
CSB-EP743012BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L20 (rplT) CSB-YP743013BIK
CSB-EP743013BIK
CSB-BP743013BIK
CSB-MP743013BIK
CSB-EP743013BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S20 (rpsT) CSB-YP743014BIK
CSB-EP743014BIK
CSB-BP743014BIK
CSB-MP743014BIK
CSB-EP743014BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18 (rpsR) CSB-YP743015BIK
CSB-EP743015BIK
CSB-BP743015BIK
CSB-MP743015BIK
CSB-EP743015BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP743016BIK
CSB-EP743016BIK
CSB-BP743016BIK
CSB-MP743016BIK
CSB-EP743016BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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