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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Leucine-rich repeat-containing protein 69 (LRRC69) CSB-YP744398HU
CSB-EP744398HU
CSB-BP744398HU
CSB-MP744398HU
CSB-EP744398HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PQ-loop repeat-containing protein 2 (PQLC2), partial CSB-YP744399HU1
CSB-EP744399HU1
CSB-BP744399HU1
CSB-MP744399HU1
CSB-EP744399HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Stress-responsive DNAJB4-interacting membrane protein 1 (SDIM1), partial CSB-YP744400HU1
CSB-EP744400HU1
CSB-BP744400HU1
CSB-MP744400HU1
CSB-EP744400HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse T-lymphoma invasion and metastasis-inducing protein 2 (Tiam2), partial CSB-YP744401MO
CSB-EP744401MO
CSB-BP744401MO
CSB-MP744401MO
CSB-EP744401MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein Jade-1 (Phf17), partial CSB-YP744402MO
CSB-EP744402MO
CSB-BP744402MO
CSB-MP744402MO
CSB-EP744402MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ATP-dependent RNA helicase DDX55 (Ddx55) CSB-YP744403MO
CSB-EP744403MO
CSB-BP744403MO
CSB-MP744403MO
CSB-EP744403MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse KIF1-binding protein (Kbp) CSB-YP744404MO
CSB-EP744404MO
CSB-BP744404MO
CSB-MP744404MO
CSB-EP744404MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho GTPase-activating protein 26 (Arhgap26), partial CSB-YP744405MO
CSB-EP744405MO
CSB-BP744405MO
CSB-MP744405MO
CSB-EP744405MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 34 (Usp34), partial CSB-YP744406MO
CSB-EP744406MO
CSB-BP744406MO
CSB-MP744406MO
CSB-EP744406MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DNA topoisomerase 2-binding protein 1 (Topbp1), partial CSB-YP744407MO
CSB-EP744407MO
CSB-BP744407MO
CSB-MP744407MO
CSB-EP744407MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 (Acap2) CSB-YP744408MO
CSB-EP744408MO
CSB-BP744408MO
CSB-MP744408MO
CSB-EP744408MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein FLJ45035 CSB-YP744409HU
CSB-EP744409HU
CSB-BP744409HU
CSB-MP744409HU
CSB-EP744409HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glutamate decarboxylase-like protein 1 (GADL1) CSB-YP744410HU
CSB-EP744410HU
CSB-BP744410HU
CSB-MP744410HU
CSB-EP744410HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Rho GTPase-activating protein 36 (ARHGAP36) CSB-YP744411HU
CSB-EP744411HU
CSB-BP744411HU
CSB-MP744411HU
CSB-EP744411HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Leucine-rich repeat-containing protein 9 (LRRC9), partial CSB-YP744412HU
CSB-EP744412HU
CSB-BP744412HU
CSB-MP744412HU
CSB-EP744412HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein FLJ45999 CSB-YP744413HU
CSB-EP744413HU
CSB-BP744413HU
CSB-MP744413HU
CSB-EP744413HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative glycerol kinase 5 (GK5) CSB-YP744414HU
CSB-EP744414HU
CSB-BP744414HU
CSB-MP744414HU
CSB-EP744414HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C16orf85 (C16orf85) CSB-YP744415HU
CSB-EP744415HU
CSB-BP744415HU
CSB-MP744415HU
CSB-EP744415HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein FLJ45252 CSB-YP744416HU
CSB-EP744416HU
CSB-BP744416HU
CSB-MP744416HU
CSB-EP744416HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Rho guanine nucleotide exchange factor 18 (ARHGEF18), partial CSB-YP744417HU
CSB-EP744417HU
CSB-BP744417HU
CSB-MP744417HU
CSB-EP744417HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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