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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Treponema denticola 30S ribosomal protein S3 (rpsC) CSB-YP744798TPP
CSB-EP744798TPP
CSB-BP744798TPP
CSB-MP744798TPP
CSB-EP744798TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP744799TPP
CSB-EP744799TPP
CSB-BP744799TPP
CSB-MP744799TPP
CSB-EP744799TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP744800TPP
CSB-EP744800TPP
CSB-BP744800TPP
CSB-MP744800TPP
CSB-EP744800TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Fructose-bisphosphate aldolase class 1 (fda) CSB-YP744801TPP
CSB-EP744801TPP
CSB-BP744801TPP
CSB-MP744801TPP
CSB-EP744801TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Pyrrolidone-carboxylate peptidase (pcp) CSB-YP744802TPP
CSB-EP744802TPP
CSB-BP744802TPP
CSB-MP744802TPP
CSB-EP744802TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Probable peroxiredoxin (TDE_0011) CSB-YP744803TPP
CSB-EP744803TPP
CSB-BP744803TPP
CSB-MP744803TPP
CSB-EP744803TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S4 (rpsD) CSB-YP744804MLA
CSB-EP744804MLA
CSB-BP744804MLA
CSB-MP744804MLA
CSB-EP744804MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase MAP_4189c (MAP_4189c) CSB-YP744805MLA
CSB-EP744805MLA
CSB-BP744805MLA
CSB-MP744805MLA
CSB-EP744805MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S14 type Z (rpsZ) CSB-YP744806MLA
CSB-EP744806MLA
CSB-BP744806MLA
CSB-MP744806MLA
CSB-EP744806MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23 (rplW) CSB-YP744807MLA
CSB-EP744807MLA
CSB-BP744807MLA
CSB-MP744807MLA
CSB-EP744807MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S12 (rpsL) CSB-YP744808MLA
CSB-EP744808MLA
CSB-BP744808MLA
CSB-MP744808MLA
CSB-EP744808MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 2 (rpmG2) CSB-YP744809MLA
CSB-EP744809MLA
CSB-BP744809MLA
CSB-MP744809MLA
CSB-EP744809MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphobilinogen deaminase (hemC) CSB-YP744810MLA
CSB-EP744810MLA
CSB-BP744810MLA
CSB-MP744810MLA
CSB-EP744810MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carboxylate-amine ligase MAP_3922 (MAP_3922) CSB-YP744811MLA
CSB-EP744811MLA
CSB-BP744811MLA
CSB-MP744811MLA
CSB-EP744811MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxycytidine triphosphate deaminase (dcd) CSB-YP744812MLA
CSB-EP744812MLA
CSB-BP744812MLA
CSB-MP744812MLA
CSB-EP744812MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized methyltransferase MAP_3663c (MAP_3663c) CSB-YP744813MLA
CSB-EP744813MLA
CSB-BP744813MLA
CSB-MP744813MLA
CSB-EP744813MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP744814MLA
CSB-EP744814MLA
CSB-BP744814MLA
CSB-MP744814MLA
CSB-EP744814MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase MAP_3385 (MAP_3385) CSB-YP744815MLA
CSB-EP744815MLA
CSB-BP744815MLA
CSB-MP744815MLA
CSB-EP744815MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP744816MLA1
CSB-EP744816MLA1
CSB-BP744816MLA1
CSB-MP744816MLA1
CSB-EP744816MLA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0061 protein MAP_3154 (MAP_3154) CSB-YP744817MLA
CSB-EP744817MLA
CSB-BP744817MLA
CSB-MP744817MLA
CSB-EP744817MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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