Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Indole-3-glycerol phosphate synthase (trpC) CSB-YP743537BAAE
CSB-EP743537BAAE
CSB-BP743537BAAE
CSB-MP743537BAAE
CSB-EP743537BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0337 protein BT9727_0908 (BT9727_0908) CSB-YP743538BAAE
CSB-EP743538BAAE
CSB-BP743538BAAE
CSB-MP743538BAAE
CSB-EP743538BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Potassium-transporting ATPase A chain (kdpA), partial CSB-YP743539BAAE
CSB-EP743539BAAE
CSB-BP743539BAAE
CSB-MP743539BAAE
CSB-EP743539BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP743540BAAE
CSB-EP743540BAAE
CSB-BP743540BAAE
CSB-MP743540BAAE
CSB-EP743540BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Methylthioribose-1-phosphate isomerase 1 (mtnA1) CSB-YP743541BAAE
CSB-EP743541BAAE
CSB-BP743541BAAE
CSB-MP743541BAAE
CSB-EP743541BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP743542BAAE
CSB-EP743542BAAE
CSB-BP743542BAAE
CSB-MP743542BAAE
CSB-EP743542BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP743543BAAE
CSB-EP743543BAAE
CSB-BP743543BAAE
CSB-MP743543BAAE
CSB-EP743543BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP743544BAAE
CSB-EP743544BAAE
CSB-BP743544BAAE
CSB-MP743544BAAE
CSB-EP743544BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP743545BAAE
CSB-EP743545BAAE
CSB-BP743545BAAE
CSB-MP743545BAAE
CSB-EP743545BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S14 type Z (rpsZ) CSB-YP743546BAAE
CSB-EP743546BAAE
CSB-BP743546BAAE
CSB-MP743546BAAE
CSB-EP743546BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L16 (rplP) CSB-YP743547BAAE
CSB-EP743547BAAE
CSB-BP743547BAAE
CSB-MP743547BAAE
CSB-EP743547BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L3 (rplC) CSB-YP743548BAAE
CSB-EP743548BAAE
CSB-BP743548BAAE
CSB-MP743548BAAE
CSB-EP743548BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S12 (rpsL) CSB-YP743549BAAE
CSB-EP743549BAAE
CSB-BP743549BAAE
CSB-MP743549BAAE
CSB-EP743549BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Peptidyl-tRNA hydrolase (pth) CSB-YP743550BAAE
CSB-EP743550BAAE
CSB-BP743550BAAE
CSB-MP743550BAAE
CSB-EP743550BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Recombination protein RecR (recR) CSB-YP743551BAAE
CSB-EP743551BAAE
CSB-BP743551BAAE
CSB-MP743551BAAE
CSB-EP743551BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian DNA replication and repair protein recF (recF) CSB-YP743552BAAE
CSB-EP743552BAAE
CSB-BP743552BAAE
CSB-MP743552BAAE
CSB-EP743552BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L32 (rpmF) CSB-YP743553BQE
CSB-EP743553BQE
CSB-BP743553BQE
CSB-MP743553BQE
CSB-EP743553BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP743554BQE
CSB-EP743554BQE
CSB-BP743554BQE
CSB-MP743554BQE
CSB-EP743554BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable indole-3-acetic acid-amido synthetase GH3.5 (GH3.5), partial CSB-YP743555OFG
CSB-EP743555OFG
CSB-BP743555OFG
CSB-MP743555OFG
CSB-EP743555OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica BURP domain-containing protein 1 (BURP1) CSB-YP743556OFG
CSB-EP743556OFG
CSB-BP743556OFG
CSB-MP743556OFG
CSB-EP743556OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>