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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gloeobacter violaceus Homoserine kinase (thrB) CSB-YP745662GCI
CSB-EP745662GCI
CSB-BP745662GCI
CSB-MP745662GCI
CSB-EP745662GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP745663GCI
CSB-EP745663GCI
CSB-BP745663GCI
CSB-MP745663GCI
CSB-EP745663GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Glycogen synthase (glgA) CSB-YP745664GCI
CSB-EP745664GCI
CSB-BP745664GCI
CSB-MP745664GCI
CSB-EP745664GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Photosystem II reaction center protein L (psbL), partial CSB-YP745665GCI
CSB-EP745665GCI
CSB-BP745665GCI
CSB-MP745665GCI
CSB-EP745665GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Ferrochelatase (hemH) CSB-YP745666GCI
CSB-EP745666GCI
CSB-BP745666GCI
CSB-MP745666GCI
CSB-EP745666GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus FO synthase subunit 2 (cofH) CSB-YP745667GCI
CSB-EP745667GCI
CSB-BP745667GCI
CSB-MP745667GCI
CSB-EP745667GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP745668GCI
CSB-EP745668GCI
CSB-BP745668GCI
CSB-MP745668GCI
CSB-EP745668GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP745669GCI
CSB-EP745669GCI
CSB-BP745669GCI
CSB-MP745669GCI
CSB-EP745669GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Potassium-transporting ATPase C chain (kdpC), partial CSB-YP745670GCI1
CSB-EP745670GCI1
CSB-BP745670GCI1
CSB-MP745670GCI1
CSB-EP745670GCI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Probable inorganic polyphosphate/ATP-NAD kinase 1 (ppnK1) CSB-YP745671GCI
CSB-EP745671GCI
CSB-BP745671GCI
CSB-MP745671GCI
CSB-EP745671GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Translation initiation factor IF-1 (infA) CSB-YP745672GCI
CSB-EP745672GCI
CSB-BP745672GCI
CSB-MP745672GCI
CSB-EP745672GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Putative ABC transporter ATP-binding protein gll0289 (gll0289) CSB-YP745673GCI
CSB-EP745673GCI
CSB-BP745673GCI
CSB-MP745673GCI
CSB-EP745673GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Undecaprenyl pyrophosphate synthase (uppS) CSB-YP745674GCI
CSB-EP745674GCI
CSB-BP745674GCI
CSB-MP745674GCI
CSB-EP745674GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP745675GCI1
CSB-EP745675GCI1
CSB-BP745675GCI1
CSB-MP745675GCI1
CSB-EP745675GCI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L34 (rpmH) CSB-YP745676CKA
CSB-EP745676CKA
CSB-BP745676CKA
CSB-MP745676CKA
CSB-EP745676CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Membrane protein insertase YidC (yidC), partial CSB-YP745677CKA1
CSB-EP745677CKA1
CSB-BP745677CKA1
CSB-MP745677CKA1
CSB-EP745677CKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP745678CKA
CSB-EP745678CKA
CSB-BP745678CKA
CSB-MP745678CKA
CSB-EP745678CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP745679CKA
CSB-EP745679CKA
CSB-BP745679CKA
CSB-MP745679CKA
CSB-EP745679CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP745680CKA
CSB-EP745680CKA
CSB-BP745680CKA
CSB-MP745680CKA
CSB-EP745680CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L7/L12 (rplL) CSB-YP745681CKA
CSB-EP745681CKA
CSB-BP745681CKA
CSB-MP745681CKA
CSB-EP745681CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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