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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serotype 4b 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP744518LAAD
CSB-EP744518LAAD
CSB-BP744518LAAD
CSB-MP744518LAAD
CSB-EP744518LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Adapter protein mecA (mecA) CSB-YP744519LAAD
CSB-EP744519LAAD
CSB-BP744519LAAD
CSB-MP744519LAAD
CSB-EP744519LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Heme A synthase (ctaA), partial CSB-YP744520LAAD
CSB-EP744520LAAD
CSB-BP744520LAAD
CSB-MP744520LAAD
CSB-EP744520LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP744521LAAD
CSB-EP744521LAAD
CSB-BP744521LAAD
CSB-MP744521LAAD
CSB-EP744521LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 3-isopropylmalate dehydrogenase (leuB) CSB-YP744522LAAD
CSB-EP744522LAAD
CSB-BP744522LAAD
CSB-MP744522LAAD
CSB-EP744522LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Ferredoxin--NADP reductase 1 (LMOf2365_1991) CSB-YP744523LAAD
CSB-EP744523LAAD
CSB-BP744523LAAD
CSB-MP744523LAAD
CSB-EP744523LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP744524LAAD
CSB-EP744524LAAD
CSB-BP744524LAAD
CSB-MP744524LAAD
CSB-EP744524LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0302 protein LMOf2365_1950 (LMOf2365_1950) CSB-YP744525LAAD
CSB-EP744525LAAD
CSB-BP744525LAAD
CSB-MP744525LAAD
CSB-EP744525LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Pantothenate synthetase (panC) CSB-YP744526LAAD
CSB-EP744526LAAD
CSB-BP744526LAAD
CSB-MP744526LAAD
CSB-EP744526LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S14 (rpsN) CSB-YP744527LAAD
CSB-EP744527LAAD
CSB-BP744527LAAD
CSB-MP744527LAAD
CSB-EP744527LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Orotate phosphoribosyltransferase (pyrE) CSB-YP744528LAAD
CSB-EP744528LAAD
CSB-BP744528LAAD
CSB-MP744528LAAD
CSB-EP744528LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative ribosome biogenesis GTPase RsgA 1 (rsgA1) CSB-YP744529LAAD
CSB-EP744529LAAD
CSB-BP744529LAAD
CSB-MP744529LAAD
CSB-EP744529LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Acyl carrier protein (acpP) CSB-YP744530LAAD
CSB-EP744530LAAD
CSB-BP744530LAAD
CSB-MP744530LAAD
CSB-EP744530LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L19 (rplS) CSB-YP744531LAAD
CSB-EP744531LAAD
CSB-BP744531LAAD
CSB-MP744531LAAD
CSB-EP744531LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Peptidase T (pepT) CSB-YP744532LAAD
CSB-EP744532LAAD
CSB-BP744532LAAD
CSB-MP744532LAAD
CSB-EP744532LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b DNA ligase (ligA), partial CSB-YP744533LAAD
CSB-EP744533LAAD
CSB-BP744533LAAD
CSB-MP744533LAAD
CSB-EP744533LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP744534LAAD
CSB-EP744534LAAD
CSB-BP744534LAAD
CSB-MP744534LAAD
CSB-EP744534LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Leucine--tRNA ligase (leuS), partial CSB-YP744535LAAD
CSB-EP744535LAAD
CSB-BP744535LAAD
CSB-MP744535LAAD
CSB-EP744535LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b D-alanine aminotransferase (dat) CSB-YP744536LAAD
CSB-EP744536LAAD
CSB-BP744536LAAD
CSB-MP744536LAAD
CSB-EP744536LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP744537LAAD
CSB-EP744537LAAD
CSB-BP744537LAAD
CSB-MP744537LAAD
CSB-EP744537LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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