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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serotype 4b Probable GTP-binding protein EngB (engB) CSB-YP744538LAAD
CSB-EP744538LAAD
CSB-BP744538LAAD
CSB-MP744538LAAD
CSB-EP744538LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glycerol kinase (glpK) CSB-YP744539LAAD
CSB-EP744539LAAD
CSB-BP744539LAAD
CSB-MP744539LAAD
CSB-EP744539LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP744540LAAD
CSB-EP744540LAAD
CSB-BP744540LAAD
CSB-MP744540LAAD
CSB-EP744540LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0473 protein LMOf2365_1520 (LMOf2365_1520) CSB-YP744541LAAD
CSB-EP744541LAAD
CSB-BP744541LAAD
CSB-MP744541LAAD
CSB-EP744541LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S20 (rpsT) CSB-YP744542LAAD
CSB-EP744542LAAD
CSB-BP744542LAAD
CSB-MP744542LAAD
CSB-EP744542LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S21 (rpsU) CSB-YP744543LAAD
CSB-EP744543LAAD
CSB-BP744543LAAD
CSB-MP744543LAAD
CSB-EP744543LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Probable manganese-dependent inorganic pyrophosphatase (ppaC) CSB-YP744544LAAD
CSB-EP744544LAAD
CSB-BP744544LAAD
CSB-MP744544LAAD
CSB-EP744544LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b DNA mismatch repair protein MutS (mutS), partial CSB-YP744545LAAD
CSB-EP744545LAAD
CSB-BP744545LAAD
CSB-MP744545LAAD
CSB-EP744545LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP744546LAAD
CSB-EP744546LAAD
CSB-BP744546LAAD
CSB-MP744546LAAD
CSB-EP744546LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b N utilization substance protein B homolog (nusB) CSB-YP744547LAAD
CSB-EP744547LAAD
CSB-BP744547LAAD
CSB-MP744547LAAD
CSB-EP744547LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP744548LAAD
CSB-EP744548LAAD
CSB-BP744548LAAD
CSB-MP744548LAAD
CSB-EP744548LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b LexA repressor (lexA) CSB-YP744549LAAD
CSB-EP744549LAAD
CSB-BP744549LAAD
CSB-MP744549LAAD
CSB-EP744549LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Gamma-glutamyl phosphate reductase (proA) CSB-YP744550LAAD
CSB-EP744550LAAD
CSB-BP744550LAAD
CSB-MP744550LAAD
CSB-EP744550LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP744551LAAD
CSB-EP744551LAAD
CSB-BP744551LAAD
CSB-MP744551LAAD
CSB-EP744551LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP744552LAAD
CSB-EP744552LAAD
CSB-BP744552LAAD
CSB-MP744552LAAD
CSB-EP744552LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b D-alanine--poly (phosphoribitol) ligase subunit 2 (dltC) CSB-YP744553LAAD
CSB-EP744553LAAD
CSB-BP744553LAAD
CSB-MP744553LAAD
CSB-EP744553LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative tRNA (cytidine (34)-2'-O)-methyltransferase (LMOf2365_0956) CSB-YP744554LAAD
CSB-EP744554LAAD
CSB-BP744554LAAD
CSB-MP744554LAAD
CSB-EP744554LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP744555LAAD
CSB-EP744555LAAD
CSB-BP744555LAAD
CSB-MP744555LAAD
CSB-EP744555LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Fructose-1,6-bisphosphatase class 3 (fbp), partial CSB-YP744556LAAD
CSB-EP744556LAAD
CSB-BP744556LAAD
CSB-MP744556LAAD
CSB-EP744556LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP744557LAAD
CSB-EP744557LAAD
CSB-BP744557LAAD
CSB-MP744557LAAD
CSB-EP744557LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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