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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Ribosome maturation factor RimM (rimM) CSB-YP744718BQN
CSB-EP744718BQN
CSB-BP744718BQN
CSB-MP744718BQN
CSB-EP744718BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP744719BQN
CSB-EP744719BQN
CSB-BP744719BQN
CSB-MP744719BQN
CSB-EP744719BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP744720BQN
CSB-EP744720BQN
CSB-BP744720BQN
CSB-MP744720BQN
CSB-EP744720BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Peptidase T (pepT) CSB-YP744721BQN
CSB-EP744721BQN
CSB-BP744721BQN
CSB-MP744721BQN
CSB-EP744721BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP744722HDAK
CSB-EP744722HDAK
CSB-BP744722HDAK
CSB-MP744722HDAK
CSB-EP744722HDAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein hfq (hfq) CSB-YP744723BQN
CSB-EP744723BQN
CSB-BP744723BQN
CSB-MP744723BQN
CSB-EP744723BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Urease accessory protein UreG (ureG) CSB-YP744724BQN
CSB-EP744724BQN
CSB-BP744724BQN
CSB-MP744724BQN
CSB-EP744724BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP744725BQN
CSB-EP744725BQN
CSB-BP744725BQN
CSB-MP744725BQN
CSB-EP744725BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable malate:quinone oxidoreductase (mqo) CSB-YP744726BQN
CSB-EP744726BQN
CSB-BP744726BQN
CSB-MP744726BQN
CSB-EP744726BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable manganese-dependent inorganic pyrophosphatase (ppaC) CSB-YP744727BQN
CSB-EP744727BQN
CSB-BP744727BQN
CSB-MP744727BQN
CSB-EP744727BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0397 protein BCE_2667 (BCE_2667), partial CSB-YP744728BQN1
CSB-EP744728BQN1
CSB-BP744728BQN1
CSB-MP744728BQN1
CSB-EP744728BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus FMN-dependent NADH-azoreductase 3 (azoR3) CSB-YP744729BQN
CSB-EP744729BQN
CSB-BP744729BQN
CSB-MP744729BQN
CSB-EP744729BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Homoserine kinase (thrB) CSB-YP744730BQN
CSB-EP744730BQN
CSB-BP744730BQN
CSB-MP744730BQN
CSB-EP744730BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0398 protein BCE_1688 (BCE_1688) CSB-YP744731BQN
CSB-EP744731BQN
CSB-BP744731BQN
CSB-MP744731BQN
CSB-EP744731BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pantothenate synthetase (panC) CSB-YP744732BQN
CSB-EP744732BQN
CSB-BP744732BQN
CSB-MP744732BQN
CSB-EP744732BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTPase Der (der) CSB-YP744733BQN
CSB-EP744733BQN
CSB-BP744733BQN
CSB-MP744733BQN
CSB-EP744733BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Flavohemoprotein (hmp) CSB-YP744734BQN
CSB-EP744734BQN
CSB-BP744734BQN
CSB-MP744734BQN
CSB-EP744734BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable multidrug resistance protein norM (norM), partial CSB-YP744735BQN
CSB-EP744735BQN
CSB-BP744735BQN
CSB-MP744735BQN
CSB-EP744735BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphonoacetaldehyde hydrolase (phnX) CSB-YP744736BQN
CSB-EP744736BQN
CSB-BP744736BQN
CSB-MP744736BQN
CSB-EP744736BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anthranilate phosphoribosyltransferase (trpD) CSB-YP744737BQN
CSB-EP744737BQN
CSB-BP744737BQN
CSB-MP744737BQN
CSB-EP744737BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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