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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP744698BQN
CSB-EP744698BQN
CSB-BP744698BQN
CSB-MP744698BQN
CSB-EP744698BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Asparagine--tRNA ligase (asnS) CSB-YP744699BQN
CSB-EP744699BQN
CSB-BP744699BQN
CSB-MP744699BQN
CSB-EP744699BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamyl-tRNA reductase (hemA) CSB-YP744700BQN
CSB-EP744700BQN
CSB-BP744700BQN
CSB-MP744700BQN
CSB-EP744700BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0758 protein BCE_4545 (BCE_4545) CSB-YP744701BQN
CSB-EP744701BQN
CSB-BP744701BQN
CSB-MP744701BQN
CSB-EP744701BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Quinolinate synthase A (nadA) CSB-YP744702BQN
CSB-EP744702BQN
CSB-BP744702BQN
CSB-MP744702BQN
CSB-EP744702BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0297 protein BCE_4470 (BCE_4470) CSB-YP744703BQN
CSB-EP744703BQN
CSB-BP744703BQN
CSB-MP744703BQN
CSB-EP744703BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Transcription elongation factor GreA (greA) CSB-YP744704BQN
CSB-EP744704BQN
CSB-BP744704BQN
CSB-MP744704BQN
CSB-EP744704BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP744705BQN
CSB-EP744705BQN
CSB-BP744705BQN
CSB-MP744705BQN
CSB-EP744705BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein grpE (grpE) CSB-YP744706BQN
CSB-EP744706BQN
CSB-BP744706BQN
CSB-MP744706BQN
CSB-EP744706BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable rRNA maturation factor (BCE_4383) CSB-YP744707BQN
CSB-EP744707BQN
CSB-BP744707BQN
CSB-MP744707BQN
CSB-EP744707BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP744708BQN
CSB-EP744708BQN
CSB-BP744708BQN
CSB-MP744708BQN
CSB-EP744708BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus L-cystine uptake protein tcyP (BCE_4324), partial CSB-YP744709BQN
CSB-EP744709BQN
CSB-BP744709BQN
CSB-MP744709BQN
CSB-EP744709BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Transcriptional regulator MntR (mntR) CSB-YP744710BQN
CSB-EP744710BQN
CSB-BP744710BQN
CSB-MP744710BQN
CSB-EP744710BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable butyrate kinase (buk) CSB-YP744711BQN
CSB-EP744711BQN
CSB-BP744711BQN
CSB-MP744711BQN
CSB-EP744711BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anti-sigma F factor (spoIIAB) CSB-YP744712BQN
CSB-EP744712BQN
CSB-BP744712BQN
CSB-MP744712BQN
CSB-EP744712BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0223 protein BCE_4008 (BCE_4008) CSB-YP744713BQN
CSB-EP744713BQN
CSB-BP744713BQN
CSB-MP744713BQN
CSB-EP744713BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0747 protein BCE_3965 (BCE_3965), partial CSB-YP744714BQN
CSB-EP744714BQN
CSB-BP744714BQN
CSB-MP744714BQN
CSB-EP744714BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Carbamoyl-phosphate synthase small chain (carA) CSB-YP744715BQN
CSB-EP744715BQN
CSB-BP744715BQN
CSB-MP744715BQN
CSB-EP744715BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Orotate phosphoribosyltransferase (pyrE) CSB-YP744716BQN
CSB-EP744716BQN
CSB-BP744716BQN
CSB-MP744716BQN
CSB-EP744716BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP744717BQN
CSB-EP744717BQN
CSB-BP744717BQN
CSB-MP744717BQN
CSB-EP744717BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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