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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bordetella bronchiseptica Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP746422BTY1
CSB-EP746422BTY1
CSB-BP746422BTY1
CSB-MP746422BTY1
CSB-EP746422BTY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 3-isopropylmalate dehydratase small subunit 2 (leuD2) CSB-YP746423BTY
CSB-EP746423BTY
CSB-BP746423BTY
CSB-MP746423BTY
CSB-EP746423BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Putative phosphotransferase BB2621 (BB2621) CSB-YP746424BTY
CSB-EP746424BTY
CSB-BP746424BTY
CSB-MP746424BTY
CSB-EP746424BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ribosome-recycling factor (frr) CSB-YP746425BTY
CSB-EP746425BTY
CSB-BP746425BTY
CSB-MP746425BTY
CSB-EP746425BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Dihydroxy-acid dehydratase 2 (ilvD2), partial CSB-YP746426BTY
CSB-EP746426BTY
CSB-BP746426BTY
CSB-MP746426BTY
CSB-EP746426BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Chaperone protein hscA homolog (hscA), partial CSB-YP746427BTY
CSB-EP746427BTY
CSB-BP746427BTY
CSB-MP746427BTY
CSB-EP746427BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Trigger factor (tig) CSB-YP746428BTY
CSB-EP746428BTY
CSB-BP746428BTY
CSB-MP746428BTY
CSB-EP746428BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP746429BTY
CSB-EP746429BTY
CSB-BP746429BTY
CSB-MP746429BTY
CSB-EP746429BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0061 protein BB2107 (BB2107) CSB-YP746430BTY
CSB-EP746430BTY
CSB-BP746430BTY
CSB-MP746430BTY
CSB-EP746430BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP746431BTY
CSB-EP746431BTY
CSB-BP746431BTY
CSB-MP746431BTY
CSB-EP746431BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 30S ribosomal protein S6 (rpsF) CSB-YP746432BTY
CSB-EP746432BTY
CSB-BP746432BTY
CSB-MP746432BTY
CSB-EP746432BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica tRNA (Ile)-lysidine synthase (tilS) CSB-YP746433BTY
CSB-EP746433BTY
CSB-BP746433BTY
CSB-MP746433BTY
CSB-EP746433BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP746434BTY
CSB-EP746434BTY
CSB-BP746434BTY
CSB-MP746434BTY
CSB-EP746434BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Probable rRNA maturation factor (BB1355) CSB-YP746435BTY
CSB-EP746435BTY
CSB-BP746435BTY
CSB-MP746435BTY
CSB-EP746435BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0178 protein BB1267 (BB1267) CSB-YP746436BTY
CSB-EP746436BTY
CSB-BP746436BTY
CSB-MP746436BTY
CSB-EP746436BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP746437BTY
CSB-EP746437BTY
CSB-BP746437BTY
CSB-MP746437BTY
CSB-EP746437BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Homogentisate 1,2-dioxygenase (hmgA) CSB-YP746438BTY
CSB-EP746438BTY
CSB-BP746438BTY
CSB-MP746438BTY
CSB-EP746438BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0176 protein BB0839 (BB0839) CSB-YP746439BTY
CSB-EP746439BTY
CSB-BP746439BTY
CSB-MP746439BTY
CSB-EP746439BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Dihydroxy-acid dehydratase 1 (ilvD1), partial CSB-YP746440BTY
CSB-EP746440BTY
CSB-BP746440BTY
CSB-MP746440BTY
CSB-EP746440BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 50S ribosomal protein L21 (rplU) CSB-YP746441BTY
CSB-EP746441BTY
CSB-BP746441BTY
CSB-MP746441BTY
CSB-EP746441BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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