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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pan troglodytes Zinc finger and SCAN domain-containing protein 21 (ZSCAN21) CSB-YP027137EQV
CSB-EP027137EQV
CSB-BP027137EQV
CSB-MP027137EQV
CSB-EP027137EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Homeobox protein goosecoid (GSC) CSB-YP009950EQV
CSB-EP009950EQV
CSB-BP009950EQV
CSB-MP009950EQV
CSB-EP009950EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes ETS translocation variant 3 (ETV3), partial CSB-YP007856EQV
CSB-EP007856EQV
CSB-BP007856EQV
CSB-MP007856EQV
CSB-EP007856EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Zinc fingers and homeoboxes protein 1 (ZHX1), partial CSB-YP026481EQV
CSB-EP026481EQV
CSB-BP026481EQV
CSB-MP026481EQV
CSB-EP026481EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Homeobox expressed in ES cells 1 (HESX1) CSB-YP010314EQV
CSB-EP010314EQV
CSB-BP010314EQV
CSB-MP010314EQV
CSB-EP010314EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Zinc finger protein with KRAB and SCAN domains 5 (ZKSCAN5), partial CSB-YP026496EQV
CSB-EP026496EQV
CSB-BP026496EQV
CSB-MP026496EQV
CSB-EP026496EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Nuclear receptor subfamily 1 group I member 3 (NR1I3) CSB-YP016049EQV
CSB-EP016049EQV
CSB-BP016049EQV
CSB-MP016049EQV
CSB-EP016049EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Zinc finger and SCAN domain-containing protein 4 (ZSCAN4) CSB-YP027141EXP
CSB-EP027141EXP
CSB-BP027141EXP
CSB-MP027141EXP
CSB-EP027141EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Homeobox protein Hox-C10 (HOXC10) CSB-YP010671EXP
CSB-EP010671EXP
CSB-BP010671EXP
CSB-MP010671EXP
CSB-EP010671EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Zinc finger and SCAN domain-containing protein 21 (ZSCAN21) CSB-YP027137EXP
CSB-EP027137EXP
CSB-BP027137EXP
CSB-MP027137EXP
CSB-EP027137EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Homeobox protein MOX-1 (MEOX1) CSB-YP013695EXP
CSB-EP013695EXP
CSB-BP013695EXP
CSB-MP013695EXP
CSB-EP013695EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Eukaryotic translation initiation factor 3 subunit A (eif3a), partial CSB-YP007543XBE
CSB-EP007543XBE
CSB-BP007543XBE
CSB-MP007543XBE
CSB-EP007543XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Molybdopterin synthase catalytic subunit (MOCS2) CSB-YP014706OFF
CSB-EP014706OFF
CSB-BP014706OFF
CSB-MP014706OFF
CSB-EP014706OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Actin-related protein 3 (ARP3) CSB-YP001249OFF
CSB-EP001249OFF
CSB-BP001249OFF
CSB-MP001249OFF
CSB-EP001249OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Cyclin-dependent kinase inhibitor 1 (KRP1) CSB-YP005086OFF
CSB-EP005086OFF
CSB-BP005086OFF
CSB-MP005086OFF
CSB-EP005086OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Cysteine protease ATG4A (ATG4A) CSB-YP002289OFF
CSB-EP002289OFF
CSB-BP002289OFF
CSB-MP002289OFF
CSB-EP002289OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Transcription initiation factor IIA subunit 2 (TFIIAy) CSB-YP010000OFF
CSB-EP010000OFF
CSB-BP010000OFF
CSB-MP010000OFF
CSB-EP010000OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Cytochrome c (CC-1) CSB-YP006328OFF
CSB-EP006328OFF
CSB-BP006328OFF
CSB-MP006328OFF
CSB-EP006328OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein arginine N-methyltransferase 7 (PRMT7), partial CSB-YP018736OFF
CSB-EP018736OFF
CSB-BP018736OFF
CSB-MP018736OFF
CSB-EP018736OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 60S ribosomal protein L11 (RPL11) CSB-YP020115OFF
CSB-EP020115OFF
CSB-BP020115OFF
CSB-MP020115OFF
CSB-EP020115OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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