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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica ATP synthase subunit alpha (atpA), partial CSB-YP002344STL
CSB-EP002344STL
CSB-BP002344STL
CSB-MP002344STL
CSB-EP002344STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP synthase subunit a (atpB), partial CSB-YP015070STL1
CSB-EP015070STL1
CSB-BP015070STL1
CSB-MP015070STL1
CSB-EP015070STL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Glucose-6-phosphate isomerase (pgi) CSB-YP009717DUT
CSB-EP009717DUT
CSB-BP009717DUT
CSB-MP009717DUT
CSB-EP009717DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582DUT
CSB-EP009582DUT
CSB-BP009582DUT
CSB-MP009582DUT
CSB-EP009582DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Peptide deformylase (def) CSB-YP017707DUT
CSB-EP017707DUT
CSB-BP017707DUT
CSB-MP017707DUT
CSB-EP017707DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Translation initiation factor IF-2 (infB), partial CSB-YP015164DUT
CSB-EP015164DUT
CSB-BP015164DUT
CSB-MP015164DUT
CSB-EP015164DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP synthase subunit alpha (atpA), partial CSB-YP002344DUT
CSB-EP002344DUT
CSB-BP002344DUT
CSB-MP002344DUT
CSB-EP002344DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum tRNA pseudouridine synthase A (truA) CSB-YP019086DUT
CSB-EP019086DUT
CSB-BP019086DUT
CSB-MP019086DUT
CSB-EP019086DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Diphthine synthase (dphB) CSB-YP007133SUP
CSB-EP007133SUP
CSB-BP007133SUP
CSB-MP007133SUP
CSB-EP007133SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Flap endonuclease 1 (fen) CSB-YP008585SUP
CSB-EP008585SUP
CSB-BP008585SUP
CSB-MP008585SUP
CSB-EP008585SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Protein pelota homolog (pelA) CSB-YP017778SUP
CSB-EP017778SUP
CSB-BP017778SUP
CSB-MP017778SUP
CSB-EP017778SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Serine hydroxymethyltransferase (glyA) CSB-YP021274SUP
CSB-EP021274SUP
CSB-BP021274SUP
CSB-MP021274SUP
CSB-EP021274SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA repair protein REV1 (REV1), partial CSB-YP019576DOA
CSB-EP019576DOA
CSB-BP019576DOA
CSB-MP019576DOA
CSB-EP019576DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter agglomerans Beta-galactosidase (lacZ), partial CSB-YP009476EKP
CSB-EP009476EKP
CSB-BP009476EKP
CSB-MP009476EKP
CSB-EP009476EKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis H/ACA ribonucleoprotein complex subunit 1 (GAR1) CSB-YP009257SXM
CSB-EP009257SXM
CSB-BP009257SXM
CSB-MP009257SXM
CSB-EP009257SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ral GTPase-activating protein subunit alpha-2 (Ralgapa2), partial CSB-YP003728MO
CSB-EP003728MO
CSB-BP003728MO
CSB-MP003728MO
CSB-EP003728MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis ATP-dependent RNA helicase DHX29 (dhx29), partial CSB-YP006872XBE
CSB-EP006872XBE
CSB-BP006872XBE
CSB-MP006872XBE
CSB-EP006872XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transcription factor MafA (mafa) CSB-YP013314DIL
CSB-EP013314DIL
CSB-BP013314DIL
CSB-MP013314DIL
CSB-EP013314DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Class A basic helix-loop-helix protein 9 (bhlha9) CSB-YP002685DIL
CSB-EP002685DIL
CSB-BP002685DIL
CSB-MP002685DIL
CSB-EP002685DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lysine-specific demethylase NO66 (no66), partial CSB-YP003168DIL
CSB-EP003168DIL
CSB-BP003168DIL
CSB-MP003168DIL
CSB-EP003168DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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