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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse A disintegrin and metalloproteinase with thrombospondin motifs 13 (Adamts13), partial CSB-YP745079MO
CSB-BP745079MO
CSB-EP745079MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein Rf1, mitochondrial (Rf1), partial CSB-YP745080OFF
CSB-EP745080OFF
CSB-BP745080OFF
CSB-MP745080OFF
CSB-EP745080OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c-550 (psbV) CSB-YP745081DZV
CSB-EP745081DZV
CSB-BP745081DZV
CSB-MP745081DZV
CSB-EP745081DZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila teissieri Histone H4 (His4) CSB-YP745082DMO
CSB-EP745082DMO
CSB-BP745082DMO
CSB-MP745082DMO
CSB-EP745082DMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PDZ domain-containing protein 4 (PDZD4) CSB-YP745083HU
CSB-EP745083HU
CSB-BP745083HU
CSB-MP745083HU
CSB-EP745083HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Kinetochore protein Nuf2 (NUF2) CSB-YP745084CH
CSB-EP745084CH
CSB-BP745084CH
CSB-MP745084CH
CSB-EP745084CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mannose-6-phosphate isomerase (PMI1) CSB-YP745085CZI
CSB-EP745085CZI
CSB-BP745085CZI
CSB-MP745085CZI
CSB-EP745085CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter xylinus Cellulose-complementing protein (ccpAX) CSB-YP745086GGT
CSB-EP745086GGT
CSB-BP745086GGT
CSB-MP745086GGT
CSB-EP745086GGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Broad bean wilt virus 1 RNA2 polyprotein (RNA2) CSB-YP745087BDAP
CSB-EP745087BDAP
CSB-BP745087BDAP
CSB-MP745087BDAP
CSB-EP745087BDAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RNA-binding protein 24 (rbm24) CSB-YP745088DIL
CSB-EP745088DIL
CSB-BP745088DIL
CSB-MP745088DIL
CSB-EP745088DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cynocephalus volans Cytochrome b (MT-CYB), partial CSB-YP745089CHAM
CSB-EP745089CHAM
CSB-BP745089CHAM
CSB-MP745089CHAM
CSB-EP745089CHAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Heparan-sulfate 6-O-sulfotransferase 2 (HS6ST2), partial CSB-YP745090CH
CSB-EP745090CH
CSB-BP745090CH
CSB-MP745090CH
CSB-EP745090CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein kinase/endoribonuclease IRE2 (ERN2), partial CSB-YP745091HU
CSB-EP745091HU
CSB-BP745091HU
CSB-MP745091HU
CSB-EP745091HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Histone H3.2 (B34) CSB-YP745092NHE
CSB-EP745092NHE
CSB-BP745092NHE
CSB-MP745092NHE
CSB-EP745092NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Histone H3.3 (H3) CSB-YP745093NHE
CSB-EP745093NHE
CSB-BP745093NHE
CSB-MP745093NHE
CSB-EP745093NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein very KIND (KNDC1), partial CSB-YP745094HU
CSB-EP745094HU
CSB-BP745094HU
CSB-MP745094HU
CSB-EP745094HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase dyrk1 (dyrk1), partial CSB-YP745095DKK
CSB-EP745095DKK
CSB-BP745095DKK
CSB-MP745095DKK
CSB-EP745095DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Mitochondrial substrate carrier family protein Q (mcfQ), partial CSB-YP745096DKK1
CSB-EP745096DKK1
CSB-BP745096DKK1
CSB-MP745096DKK1
CSB-EP745096DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe ATP-dependent RNA helicase dbp6 (dbp6), partial CSB-YP745097SXV
CSB-EP745097SXV
CSB-BP745097SXV
CSB-MP745097SXV
CSB-EP745097SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Thymidylate kinase (TMK) CSB-YP745098VEE
CSB-EP745098VEE
CSB-BP745098VEE
CSB-MP745098VEE
CSB-EP745098VEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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