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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chromobacterium violaceum 30S ribosomal protein S7 (rpsG) CSB-YP745682CKA
CSB-EP745682CKA
CSB-BP745682CKA
CSB-MP745682CKA
CSB-EP745682CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L22 (rplV) CSB-YP745683CKA
CSB-EP745683CKA
CSB-BP745683CKA
CSB-MP745683CKA
CSB-EP745683CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S8 (rpsH) CSB-YP745684CKA
CSB-EP745684CKA
CSB-BP745684CKA
CSB-MP745684CKA
CSB-EP745684CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S13 (rpsM) CSB-YP745685CKA
CSB-EP745685CKA
CSB-BP745685CKA
CSB-MP745685CKA
CSB-EP745685CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP745686CKA
CSB-EP745686CKA
CSB-BP745686CKA
CSB-MP745686CKA
CSB-EP745686CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP745687CKA
CSB-EP745687CKA
CSB-BP745687CKA
CSB-MP745687CKA
CSB-EP745687CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0301 protein CV_3909 (CV_3909) CSB-YP745688CKA
CSB-EP745688CKA
CSB-BP745688CKA
CSB-MP745688CKA
CSB-EP745688CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Aspartate--tRNA ligase (aspS), partial CSB-YP745689CKA
CSB-EP745689CKA
CSB-BP745689CKA
CSB-MP745689CKA
CSB-EP745689CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L13 (rplM) CSB-YP745690CKA
CSB-EP745690CKA
CSB-BP745690CKA
CSB-MP745690CKA
CSB-EP745690CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribosome maturation factor RimM (rimM) CSB-YP745691CKA
CSB-EP745691CKA
CSB-BP745691CKA
CSB-MP745691CKA
CSB-EP745691CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Uracil phosphoribosyltransferase (upp) CSB-YP745692CKA
CSB-EP745692CKA
CSB-BP745692CKA
CSB-MP745692CKA
CSB-EP745692CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0225 protein CV_3559 (CV_3559) CSB-YP745693CKA
CSB-EP745693CKA
CSB-BP745693CKA
CSB-MP745693CKA
CSB-EP745693CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP745694CKA
CSB-EP745694CKA
CSB-BP745694CKA
CSB-MP745694CKA
CSB-EP745694CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0125 protein CV_3462 (CV_3462) CSB-YP745695CKA
CSB-EP745695CKA
CSB-BP745695CKA
CSB-MP745695CKA
CSB-EP745695CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Phosphate acyltransferase (plsX) CSB-YP745696CKA
CSB-EP745696CKA
CSB-BP745696CKA
CSB-MP745696CKA
CSB-EP745696CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0434 protein CV_3345 (CV_3345) CSB-YP745697CKA
CSB-EP745697CKA
CSB-BP745697CKA
CSB-MP745697CKA
CSB-EP745697CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0307 protein CV_3198 (CV_3198) CSB-YP745698CKA
CSB-EP745698CKA
CSB-BP745698CKA
CSB-MP745698CKA
CSB-EP745698CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UvrABC system protein B (uvrB), partial CSB-YP745699CKA
CSB-EP745699CKA
CSB-BP745699CKA
CSB-MP745699CKA
CSB-EP745699CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Cytidylate kinase (cmk) CSB-YP745700CKA
CSB-EP745700CKA
CSB-BP745700CKA
CSB-MP745700CKA
CSB-EP745700CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum GMP reductase (guaC) CSB-YP745701CKA
CSB-EP745701CKA
CSB-BP745701CKA
CSB-MP745701CKA
CSB-EP745701CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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