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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial CSB-YP745159SUL1
CSB-EP745159SUL1
CSB-BP745159SUL1
CSB-MP745159SUL1
CSB-EP745159SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator sarR (sarR) CSB-YP745160SKX
CSB-EP745160SKX
CSB-BP745160SKX
CSB-MP745160SKX
CSB-EP745160SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splB (splB) CSB-YP745161SKX
CSB-EP745161SKX
CSB-BP745161SKX
CSB-MP745161SKX
CSB-EP745161SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP745162SKX
CSB-EP745162SKX
CSB-BP745162SKX
CSB-MP745162SKX
CSB-EP745162SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein vraX (vraX) CSB-YP745163SKX
CSB-EP745163SKX
CSB-BP745163SKX
CSB-MP745163SKX
CSB-EP745163SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0397 protein SA2477 (SA2477), partial CSB-YP745164SKY1
CSB-EP745164SKY1
CSB-BP745164SKY1
CSB-MP745164SKY1
CSB-EP745164SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Accessory Sec system protein translocase subunit SecY2 (secY2), partial CSB-YP745165SKY
CSB-EP745165SKY
CSB-BP745165SKY
CSB-MP745165SKY
CSB-EP745165SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus O-acetyltransferase OatA (oatA), partial CSB-YP745166SKY
CSB-EP745166SKY
CSB-BP745166SKY
CSB-MP745166SKY
CSB-EP745166SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Copper-exporting P-type ATPase A (copA), partial CSB-YP745167SKY
CSB-EP745167SKY
CSB-BP745167SKY
CSB-MP745167SKY
CSB-EP745167SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator sarT (sarT) CSB-YP745168SKY
CSB-EP745168SKY
CSB-BP745168SKY
CSB-MP745168SKY
CSB-EP745168SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein flp (flp), partial CSB-YP745169SKY1
CSB-EP745169SKY1
CSB-BP745169SKY1
CSB-MP745169SKY1
CSB-EP745169SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized HTH-type transcriptional regulator SA2153 (SA2153) CSB-YP745170SKY
CSB-EP745170SKY
CSB-BP745170SKY
CSB-MP745170SKY
CSB-EP745170SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator sarR (sarR) CSB-YP745171SKY
CSB-EP745171SKY
CSB-BP745171SKY
CSB-MP745171SKY
CSB-EP745171SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L23 (rplW) CSB-YP745172SKY
CSB-EP745172SKY
CSB-BP745172SKY
CSB-MP745172SKY
CSB-EP745172SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L17 (rplQ) CSB-YP745173SKY
CSB-EP745173SKY
CSB-BP745173SKY
CSB-MP745173SKY
CSB-EP745173SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable uridylyltransferase SA1974 (SA1974) CSB-YP745174SKY
CSB-EP745174SKY
CSB-BP745174SKY
CSB-MP745174SKY
CSB-EP745174SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit b (atpF), partial CSB-YP745175SKY1
CSB-EP745175SKY1
CSB-BP745175SKY1
CSB-MP745175SKY1
CSB-EP745175SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus mRNA interferase MazF (mazF) CSB-YP745176SKY
CSB-EP745176SKY
CSB-BP745176SKY
CSB-MP745176SKY
CSB-EP745176SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA ligase (ligA), partial CSB-YP745177SKY
CSB-EP745177SKY
CSB-BP745177SKY
CSB-MP745177SKY
CSB-EP745177SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0435 protein SA1696 (SA1696) CSB-YP745178SKY
CSB-EP745178SKY
CSB-BP745178SKY
CSB-MP745178SKY
CSB-EP745178SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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