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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Probable NAD (P)H-dependent oxidoreductase 1 (Os10g0113000, LOC_Os10g02380) CSB-YP745279OFG
CSB-EP745279OFG
CSB-BP745279OFG
CSB-MP745279OFG
CSB-EP745279OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter C family member 5 (ABCC5), partial CSB-YP745280DOA
CSB-EP745280DOA
CSB-BP745280DOA
CSB-MP745280DOA
CSB-EP745280DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP745281FEC
CSB-EP745281FEC
CSB-BP745281FEC
CSB-MP745281FEC
CSB-EP745281FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax quinquefolius Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP745282PGAY
CSB-EP745282PGAY
CSB-BP745282PGAY
CSB-MP745282PGAY
CSB-EP745282PGAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus koraiensis 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP745283PYR
CSB-EP745283PYR
CSB-BP745283PYR
CSB-MP745283PYR
CSB-EP745283PYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psathyrostachys juncea DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP745284PGAZ
CSB-EP745284PGAZ
CSB-BP745284PGAZ
CSB-MP745284PGAZ
CSB-EP745284PGAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta pentagona Cytochrome b559 subunit beta (psbF), partial CSB-YP745285CHAN
CSB-EP745285CHAN
CSB-BP745285CHAN
CSB-MP745285CHAN
CSB-EP745285CHAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calystegia sepium Photosystem II reaction center protein L (psbL), partial CSB-YP745286CHAO
CSB-EP745286CHAO
CSB-BP745286CHAO
CSB-MP745286CHAO
CSB-EP745286CHAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea setosa Photosystem II reaction center protein L (psbL), partial CSB-YP745287IBAX
CSB-EP745287IBAX
CSB-BP745287IBAX
CSB-MP745287IBAX
CSB-EP745287IBAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea wrightii Photosystem II reaction center protein L (psbL), partial CSB-YP745288IBAW
CSB-EP745288IBAW
CSB-BP745288IBAW
CSB-MP745288IBAW
CSB-EP745288IBAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acrocomia aculeata ATP synthase subunit beta, chloroplastic (atpB) CSB-YP745289AFAJ
CSB-EP745289AFAJ
CSB-BP745289AFAJ
CSB-MP745289AFAJ
CSB-EP745289AFAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spathiphyllum wallisii Photosystem II reaction center protein L (psbL), partial CSB-YP745290SGAB
CSB-EP745290SGAB
CSB-BP745290SGAB
CSB-MP745290SGAB
CSB-EP745290SGAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sagittaria latifolia Cytochrome b559 subunit beta (psbF), partial CSB-YP745291SFAT
CSB-EP745291SFAT
CSB-BP745291SFAT
CSB-MP745291SFAT
CSB-EP745291SFAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gunnera chilensis Cytochrome b559 subunit beta (psbF), partial CSB-YP745292GDU
CSB-EP745292GDU
CSB-BP745292GDU
CSB-MP745292GDU
CSB-EP745292GDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP745293CBM
CSB-EP745293CBM
CSB-BP745293CBM
CSB-MP745293CBM
CSB-EP745293CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovis canadensis NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP745294OAAI1
CSB-EP745294OAAI1
CSB-BP745294OAAI1
CSB-MP745294OAAI1
CSB-EP745294OAAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mentzelia lindleyi Maturase K (matK), partial CSB-YP745295MFAM
CSB-EP745295MFAM
CSB-BP745295MFAM
CSB-MP745295MFAM
CSB-EP745295MFAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Larix laricina Maturase K (matK), partial CSB-YP745296LCG
CSB-EP745296LCG
CSB-BP745296LCG
CSB-MP745296LCG
CSB-EP745296LCG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propithecus tattersalli NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP745297PSJ1
CSB-EP745297PSJ1
CSB-BP745297PSJ1
CSB-MP745297PSJ1
CSB-EP745297PSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea odorata Protein psbN (psbN), partial CSB-YP745298NIE
CSB-EP745298NIE
CSB-BP745298NIE
CSB-MP745298NIE
CSB-EP745298NIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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