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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus UPF0741 protein SA0550 (SA0550) CSB-YP745199SKY
CSB-EP745199SKY
CSB-BP745199SKY
CSB-MP745199SKY
CSB-EP745199SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine-aspartate repeat-containing protein C (sdrC), partial CSB-YP745200SKY
CSB-EP745200SKY
CSB-BP745200SKY
CSB-MP745200SKY
CSB-EP745200SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcriptional regulator CtsR (ctsR) CSB-YP745201SKY
CSB-EP745201SKY
CSB-BP745201SKY
CSB-MP745201SKY
CSB-EP745201SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N-acetylmuramoyl-L-alanine amidase sle1 (sle1) CSB-YP745202SKY
CSB-EP745202SKY
CSB-BP745202SKY
CSB-MP745202SKY
CSB-EP745202SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized lipoprotein SA0398 (lpl3) CSB-YP745203SKY
CSB-EP745203SKY
CSB-BP745203SKY
CSB-MP745203SKY
CSB-EP745203SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Efem/EfeO family lipoprotein (SA0331) CSB-YP745204SKY
CSB-EP745204SKY
CSB-BP745204SKY
CSB-MP745204SKY
CSB-EP745204SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein essA (essA), partial CSB-YP745205SKY
CSB-EP745205SKY
CSB-BP745205SKY
CSB-MP745205SKY
CSB-EP745205SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP745206SKY
CSB-EP745206SKY
CSB-BP745206SKY
CSB-MP745206SKY
CSB-EP745206SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized HTH-type transcriptional regulator SA0097 (SA0097), partial CSB-YP745207SKY
CSB-EP745207SKY
CSB-BP745207SKY
CSB-MP745207SKY
CSB-EP745207SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcriptional regulatory protein walR (walR) CSB-YP745208SKY
CSB-EP745208SKY
CSB-BP745208SKY
CSB-MP745208SKY
CSB-EP745208SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulator hosA (hosA) CSB-YP745209EOD
CSB-EP745209EOD
CSB-BP745209EOD
CSB-MP745209EOD
CSB-EP745209EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP745210EOD
CSB-EP745210EOD
CSB-BP745210EOD
CSB-MP745210EOD
CSB-EP745210EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Curved DNA-binding protein (cbpA) CSB-YP745211EOD
CSB-EP745211EOD
CSB-BP745211EOD
CSB-MP745211EOD
CSB-EP745211EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP745212FOB
CSB-EP745212FOB
CSB-BP745212FOB
CSB-MP745212FOB
CSB-EP745212FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a 50S ribosomal protein L22 (rplV) CSB-YP745213LGU
CSB-EP745213LGU
CSB-BP745213LGU
CSB-MP745213LGU
CSB-EP745213LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a 30S ribosomal protein S15 (rpsO) CSB-YP745214LGU
CSB-EP745214LGU
CSB-BP745214LGU
CSB-MP745214LGU
CSB-EP745214LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional AAC/APH (aacA-aphD) CSB-YP745215SGAI
CSB-EP745215SGAI
CSB-BP745215SGAI
CSB-MP745215SGAI
CSB-EP745215SGAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri Outer membrane protein IcsA autotransporter (icsA), partial CSB-YP745216SZB
CSB-EP745216SZB
CSB-BP745216SZB
CSB-MP745216SZB
CSB-EP745216SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lanthionine-containing peptide sapB (ramS) CSB-YP745217FOI
CSB-EP745217FOI
CSB-BP745217FOI
CSB-MP745217FOI
CSB-EP745217FOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized endonuclease (L7003, ECO57PM65) CSB-YP745218EOD
CSB-EP745218EOD
CSB-BP745218EOD
CSB-MP745218EOD
CSB-EP745218EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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