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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Glutathione S-transferase A5 (GSTA5) CSB-YP745762HU
CSB-EP745762HU
CSB-BP745762HU
CSB-MP745762HU
CSB-EP745762HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cation channel sperm-associated protein 4 (CATSPER4), partial CSB-YP745763HU1
CSB-EP745763HU1
CSB-BP745763HU1
CSB-MP745763HU1
CSB-EP745763HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protease serine 7 (TMPRSS7), partial CSB-YP745764HU
CSB-EP745764HU
CSB-BP745764HU
CSB-MP745764HU
CSB-EP745764HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa GTP-binding nuclear protein GSP1/Ran (gsp-1) CSB-YP745765NHA
CSB-EP745765NHA
CSB-BP745765NHA
CSB-MP745765NHA
CSB-EP745765NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Palmitoyltransferase SWF1 (swf-1), partial CSB-YP745766NHA1
CSB-EP745766NHA1
CSB-BP745766NHA1
CSB-MP745766NHA1
CSB-EP745766NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP-dependent DNA helicase II subunit 2 (ku80), partial CSB-YP745767NHA
CSB-EP745767NHA
CSB-BP745767NHA
CSB-MP745767NHA
CSB-EP745767NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Eukaryotic translation initiation factor 3 subunit I (tif-34) CSB-YP745768NHA
CSB-EP745768NHA
CSB-BP745768NHA
CSB-MP745768NHA
CSB-EP745768NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Vacuolar protein 8 (vac-8), partial CSB-YP745769NHA
CSB-EP745769NHA
CSB-BP745769NHA
CSB-MP745769NHA
CSB-EP745769NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Probable tRNA-splicing endonuclease subunit sen-15 (sen-15) CSB-YP745770NHA
CSB-EP745770NHA
CSB-BP745770NHA
CSB-MP745770NHA
CSB-EP745770NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondrial inner membrane organizing system protein NCU06495 (NCU06495), partial CSB-YP745771NHA1
CSB-EP745771NHA1
CSB-BP745771NHA1
CSB-MP745771NHA1
CSB-EP745771NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Histone acetyltransferase type B catalytic subunit (hat-1), partial CSB-YP745772NHA
CSB-EP745772NHA
CSB-BP745772NHA
CSB-MP745772NHA
CSB-EP745772NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondrial Rho GTPase 1 (gem-1), partial CSB-YP745773NHA
CSB-EP745773NHA
CSB-BP745773NHA
CSB-MP745773NHA
CSB-EP745773NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-mRNA-splicing factor syf-2 (syf-2) CSB-YP745774NHA
CSB-EP745774NHA
CSB-BP745774NHA
CSB-MP745774NHA
CSB-EP745774NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Calpain-like protease palB/rim-13 (rim-13), partial CSB-YP745775NHA
CSB-EP745775NHA
CSB-BP745775NHA
CSB-MP745775NHA
CSB-EP745775NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Autophagy-related protein 11 (atg-11), partial CSB-YP745776NHA
CSB-EP745776NHA
CSB-BP745776NHA
CSB-MP745776NHA
CSB-EP745776NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa UPF0499 protein NCU07353 (NCU07353) CSB-YP745777NHA
CSB-EP745777NHA
CSB-BP745777NHA
CSB-MP745777NHA
CSB-EP745777NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Topoisomerase 1-associated factor 1 (tof-1), partial CSB-YP745778NHA
CSB-EP745778NHA
CSB-BP745778NHA
CSB-MP745778NHA
CSB-EP745778NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein fyv-10 (fyv-10) CSB-YP745779NHA
CSB-EP745779NHA
CSB-BP745779NHA
CSB-MP745779NHA
CSB-EP745779NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondrial intermembrane space import and assembly protein 40 (mia-40), partial CSB-YP745780NHA1
CSB-EP745780NHA1
CSB-BP745780NHA1
CSB-MP745780NHA1
CSB-EP745780NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Autophagy-related protein 9 (atg-9), partial CSB-YP745781NHA
CSB-EP745781NHA
CSB-BP745781NHA
CSB-MP745781NHA
CSB-EP745781NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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