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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Glutamyl-tRNA reductase (hemA) CSB-YP745402WBQ
CSB-EP745402WBQ
CSB-BP745402WBQ
CSB-MP745402WBQ
CSB-EP745402WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP745403VCQ
CSB-EP745403VCQ
CSB-BP745403VCQ
CSB-MP745403VCQ
CSB-EP745403VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal protein S6 modification protein (rimK) CSB-YP745404VCQ
CSB-EP745404VCQ
CSB-BP745404VCQ
CSB-MP745404VCQ
CSB-EP745404VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Periplasmic nitrate reductase (napA), partial CSB-YP745405VCQ
CSB-EP745405VCQ
CSB-BP745405VCQ
CSB-MP745405VCQ
CSB-EP745405VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0312 protein VVA0736 (VVA0736) CSB-YP745406VCQ
CSB-EP745406VCQ
CSB-BP745406VCQ
CSB-MP745406VCQ
CSB-EP745406VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Methylglyoxal synthase (mgsA) CSB-YP745407VCQ
CSB-EP745407VCQ
CSB-BP745407VCQ
CSB-MP745407VCQ
CSB-EP745407VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Dihydroorotase (pyrC) CSB-YP745408VCQ
CSB-EP745408VCQ
CSB-BP745408VCQ
CSB-MP745408VCQ
CSB-EP745408VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus L-threonine 3-dehydrogenase (tdh) CSB-YP745409VCQ
CSB-EP745409VCQ
CSB-BP745409VCQ
CSB-MP745409VCQ
CSB-EP745409VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Nitric oxide reductase FlRd-NAD (+) reductase (norW) CSB-YP745410VCQ
CSB-EP745410VCQ
CSB-BP745410VCQ
CSB-MP745410VCQ
CSB-EP745410VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative ribosome biogenesis GTPase RsgA 2 (rsgA2) CSB-YP745411VCQ
CSB-EP745411VCQ
CSB-BP745411VCQ
CSB-MP745411VCQ
CSB-EP745411VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP synthase subunit delta (atpH) CSB-YP745412VCQ
CSB-EP745412VCQ
CSB-BP745412VCQ
CSB-MP745412VCQ
CSB-EP745412VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Methionyl-tRNA formyltransferase (fmt) CSB-YP745413VCQ
CSB-EP745413VCQ
CSB-BP745413VCQ
CSB-MP745413VCQ
CSB-EP745413VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus N5-carboxyaminoimidazole ribonucleotide mutase (purE) CSB-YP745414VCQ
CSB-EP745414VCQ
CSB-BP745414VCQ
CSB-MP745414VCQ
CSB-EP745414VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP-dependent RNA helicase rhlB (rhlB) CSB-YP745415VCQ
CSB-EP745415VCQ
CSB-BP745415VCQ
CSB-MP745415VCQ
CSB-EP745415VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-dehydroquinate dehydratase (aroQ) CSB-YP745416VCQ
CSB-EP745416VCQ
CSB-BP745416VCQ
CSB-MP745416VCQ
CSB-EP745416VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP745417VCQ
CSB-EP745417VCQ
CSB-BP745417VCQ
CSB-MP745417VCQ
CSB-EP745417VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus HTH-type transcriptional repressor NsrR (nsrR) CSB-YP745418VCQ
CSB-EP745418VCQ
CSB-BP745418VCQ
CSB-MP745418VCQ
CSB-EP745418VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein SlyX homolog (slyX) CSB-YP745419VCQ
CSB-EP745419VCQ
CSB-BP745419VCQ
CSB-MP745419VCQ
CSB-EP745419VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Triosephosphate isomerase (tpiA) CSB-YP745420VCQ
CSB-EP745420VCQ
CSB-BP745420VCQ
CSB-MP745420VCQ
CSB-EP745420VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acetylornithine deacetylase (argE) CSB-YP745421VCQ
CSB-EP745421VCQ
CSB-BP745421VCQ
CSB-MP745421VCQ
CSB-EP745421VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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