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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bordetella bronchiseptica tRNA 2-selenouridine synthase (selU) CSB-YP746402BTY
CSB-EP746402BTY
CSB-BP746402BTY
CSB-MP746402BTY
CSB-EP746402BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP746403BTY
CSB-EP746403BTY
CSB-BP746403BTY
CSB-MP746403BTY
CSB-EP746403BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0042 nucleotide-binding protein BB4511 (BB4511) CSB-YP746404BTY
CSB-EP746404BTY
CSB-BP746404BTY
CSB-MP746404BTY
CSB-EP746404BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Pantothenate synthetase (panC) CSB-YP746405BTY
CSB-EP746405BTY
CSB-BP746405BTY
CSB-MP746405BTY
CSB-EP746405BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Bis (5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) CSB-YP746406BTY
CSB-EP746406BTY
CSB-BP746406BTY
CSB-MP746406BTY
CSB-EP746406BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0307 protein BB4360 (BB4360) CSB-YP746407BTY
CSB-EP746407BTY
CSB-BP746407BTY
CSB-MP746407BTY
CSB-EP746407BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Transcriptional repressor NrdR (nrdR) CSB-YP746408BTY
CSB-EP746408BTY
CSB-BP746408BTY
CSB-MP746408BTY
CSB-EP746408BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica FMN-dependent NADH-azoreductase (azoR) CSB-YP746409BTY
CSB-EP746409BTY
CSB-BP746409BTY
CSB-MP746409BTY
CSB-EP746409BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP746410BTY
CSB-EP746410BTY
CSB-BP746410BTY
CSB-MP746410BTY
CSB-EP746410BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP746411BTY
CSB-EP746411BTY
CSB-BP746411BTY
CSB-MP746411BTY
CSB-EP746411BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Peptide deformylase 2 (def2) CSB-YP746412BTY
CSB-EP746412BTY
CSB-BP746412BTY
CSB-MP746412BTY
CSB-EP746412BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Glutamine--tRNA ligase (glnS), partial CSB-YP746413BTY
CSB-EP746413BTY
CSB-BP746413BTY
CSB-MP746413BTY
CSB-EP746413BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP746414BTY
CSB-EP746414BTY
CSB-BP746414BTY
CSB-MP746414BTY
CSB-EP746414BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0391 membrane protein BB3587 (BB3587), partial CSB-YP746415BTY
CSB-EP746415BTY
CSB-BP746415BTY
CSB-MP746415BTY
CSB-EP746415BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ribose-5-phosphate isomerase A (rpiA) CSB-YP746416BTY
CSB-EP746416BTY
CSB-BP746416BTY
CSB-MP746416BTY
CSB-EP746416BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP746417BTY
CSB-EP746417BTY
CSB-BP746417BTY
CSB-MP746417BTY
CSB-EP746417BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Protein CrcB homolog (crcB), partial CSB-YP746418BTY1
CSB-EP746418BTY1
CSB-BP746418BTY1
CSB-MP746418BTY1
CSB-EP746418BTY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica L-2,4-diaminobutyric acid acetyltransferase (ectA) CSB-YP746419BTY
CSB-EP746419BTY
CSB-BP746419BTY
CSB-MP746419BTY
CSB-EP746419BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ribosome maturation factor RimM (rimM) CSB-YP746420BTY
CSB-EP746420BTY
CSB-BP746420BTY
CSB-MP746420BTY
CSB-EP746420BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Histidinol-phosphate aminotransferase 1 (hisC1) CSB-YP746421BTY
CSB-EP746421BTY
CSB-BP746421BTY
CSB-MP746421BTY
CSB-EP746421BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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