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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Picrophilus torridus Probable exosome complex RNA-binding protein 1 (PTO0395) CSB-YP747004PAAB
CSB-EP747004PAAB
CSB-BP747004PAAB
CSB-MP747004PAAB
CSB-EP747004PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus tRNA pseudouridine synthase Pus10 (pus10) CSB-YP747005PAAB
CSB-EP747005PAAB
CSB-BP747005PAAB
CSB-MP747005PAAB
CSB-EP747005PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Tryptophan synthase beta chain (trpB) CSB-YP747006PAAB
CSB-EP747006PAAB
CSB-BP747006PAAB
CSB-MP747006PAAB
CSB-EP747006PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable porphobilinogen deaminase (hemC) CSB-YP747007PAAB
CSB-EP747007PAAB
CSB-BP747007PAAB
CSB-MP747007PAAB
CSB-EP747007PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L39e (rpl39e) CSB-YP747008PAAB
CSB-EP747008PAAB
CSB-BP747008PAAB
CSB-MP747008PAAB
CSB-EP747008PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable imidazolonepropionase (hutI) CSB-YP747009PAAB
CSB-EP747009PAAB
CSB-BP747009PAAB
CSB-MP747009PAAB
CSB-EP747009PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP747010PAAB
CSB-EP747010PAAB
CSB-BP747010PAAB
CSB-MP747010PAAB
CSB-EP747010PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid Cytochrome b6 (petB), partial CSB-YP747011SAN1
CSB-EP747011SAN1
CSB-BP747011SAN1
CSB-MP747011SAN1
CSB-EP747011SAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP747012SAN
CSB-EP747012SAN
CSB-BP747012SAN
CSB-MP747012SAN
CSB-EP747012SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid ATP synthase epsilon chain, chloroplastic (atpE) CSB-YP747013SAN
CSB-EP747013SAN
CSB-BP747013SAN
CSB-MP747013SAN
CSB-EP747013SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP747014SAN
CSB-EP747014SAN
CSB-BP747014SAN
CSB-MP747014SAN
CSB-EP747014SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid Uncharacterized protein ycf72 (ycf72-1) CSB-YP747015SAN
CSB-EP747015SAN
CSB-BP747015SAN
CSB-MP747015SAN
CSB-EP747015SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid NAD (P)H-quinone oxidoreductase subunit H, chloroplastic (ndhH) CSB-YP747016SAN
CSB-EP747016SAN
CSB-BP747016SAN
CSB-MP747016SAN
CSB-EP747016SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid NAD (P)H-quinone oxidoreductase chain 4, chloroplastic (ndhD), partial CSB-YP747017SAN
CSB-EP747017SAN
CSB-BP747017SAN
CSB-MP747017SAN
CSB-EP747017SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid NAD (P)H-quinone oxidoreductase subunit 5, chloroplastic (ndhF), partial CSB-YP747018SAN
CSB-EP747018SAN
CSB-BP747018SAN
CSB-MP747018SAN
CSB-EP747018SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP747019SAN
CSB-EP747019SAN
CSB-BP747019SAN
CSB-MP747019SAN
CSB-EP747019SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum demissum Putative late blight resistance protein homolog R1A-3 (R1A-3), partial CSB-YP747020SZW
CSB-EP747020SZW
CSB-BP747020SZW
CSB-MP747020SZW
CSB-EP747020SZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 52 (Os05g0587100, LOC_Os05g50970) CSB-YP747021OFG
CSB-EP747021OFG
CSB-BP747021OFG
CSB-MP747021OFG
CSB-EP747021OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative auxin efflux carrier component 3b (PIN3B), partial CSB-YP747022OFG
CSB-EP747022OFG
CSB-BP747022OFG
CSB-MP747022OFG
CSB-EP747022OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Receptor polysaccharide phosphotransferase wefF (wefF) CSB-YP747023FMP
CSB-EP747023FMP
CSB-BP747023FMP
CSB-MP747023FMP
CSB-EP747023FMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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