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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopirellula baltica Indole-3-glycerol phosphate synthase (trpC) CSB-YP746002RDR
CSB-EP746002RDR
CSB-BP746002RDR
CSB-MP746002RDR
CSB-EP746002RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 50S ribosomal protein L9 (rplI) CSB-YP746003RDR
CSB-EP746003RDR
CSB-BP746003RDR
CSB-MP746003RDR
CSB-EP746003RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Polyphosphate kinase (ppk), partial CSB-YP746004RDR
CSB-EP746004RDR
CSB-BP746004RDR
CSB-MP746004RDR
CSB-EP746004RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP746005RDR
CSB-EP746005RDR
CSB-BP746005RDR
CSB-MP746005RDR
CSB-EP746005RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Chaperone protein ClpB (clpB), partial CSB-YP746006RDR
CSB-EP746006RDR
CSB-BP746006RDR
CSB-MP746006RDR
CSB-EP746006RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Leucine--tRNA ligase (leuS), partial CSB-YP746007RDR
CSB-EP746007RDR
CSB-BP746007RDR
CSB-MP746007RDR
CSB-EP746007RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 30S ribosomal protein S5 (rpsE) CSB-YP746008RDR
CSB-EP746008RDR
CSB-BP746008RDR
CSB-MP746008RDR
CSB-EP746008RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 50S ribosomal protein L24 (rplX) CSB-YP746009RDR
CSB-EP746009RDR
CSB-BP746009RDR
CSB-MP746009RDR
CSB-EP746009RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 50S ribosomal protein L3 (rplC) CSB-YP746010RDR
CSB-EP746010RDR
CSB-BP746010RDR
CSB-MP746010RDR
CSB-EP746010RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Histidinol-phosphate aminotransferase (hisC) CSB-YP746011RDR
CSB-EP746011RDR
CSB-BP746011RDR
CSB-MP746011RDR
CSB-EP746011RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Bifunctional protein FolD (folD) CSB-YP746012RDR
CSB-EP746012RDR
CSB-BP746012RDR
CSB-MP746012RDR
CSB-EP746012RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Isoleucine--tRNA ligase (ileS), partial CSB-YP746013RDR
CSB-EP746013RDR
CSB-BP746013RDR
CSB-MP746013RDR
CSB-EP746013RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP746014RDR
CSB-EP746014RDR
CSB-BP746014RDR
CSB-MP746014RDR
CSB-EP746014RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Lipoprotein-releasing system ATP-binding protein LolD 2 (lolD2) CSB-YP746015RDR
CSB-EP746015RDR
CSB-BP746015RDR
CSB-MP746015RDR
CSB-EP746015RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Deoxyribose-phosphate aldolase (deoC) CSB-YP746016RDR
CSB-EP746016RDR
CSB-BP746016RDR
CSB-MP746016RDR
CSB-EP746016RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP746017RDR
CSB-EP746017RDR
CSB-BP746017RDR
CSB-MP746017RDR
CSB-EP746017RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Chromosome partition protein Smc (smc), partial CSB-YP746018RDR
CSB-EP746018RDR
CSB-BP746018RDR
CSB-MP746018RDR
CSB-EP746018RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Ribonuclease PH (rph) CSB-YP746019RDR
CSB-EP746019RDR
CSB-BP746019RDR
CSB-MP746019RDR
CSB-EP746019RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Tryptophan synthase alpha chain (trpA) CSB-YP746020RDR
CSB-EP746020RDR
CSB-BP746020RDR
CSB-MP746020RDR
CSB-EP746020RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Elongation factor G (fusA), partial CSB-YP746021RDR
CSB-EP746021RDR
CSB-BP746021RDR
CSB-MP746021RDR
CSB-EP746021RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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