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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus subsp. pastoris Tyrosine--tRNA ligase (tyrS) CSB-YP746042EYQ
CSB-EP746042EYQ
CSB-BP746042EYQ
CSB-MP746042EYQ
CSB-EP746042EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Thiamine-phosphate synthase (thiE) CSB-YP746043EYQ
CSB-EP746043EYQ
CSB-BP746043EYQ
CSB-MP746043EYQ
CSB-EP746043EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Membrane protein insertase YidC (yidC), partial CSB-YP746044EYQ1
CSB-EP746044EYQ1
CSB-BP746044EYQ1
CSB-MP746044EYQ1
CSB-EP746044EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ribose-phosphate pyrophosphokinase (prs) CSB-YP746045EYQ
CSB-EP746045EYQ
CSB-BP746045EYQ
CSB-MP746045EYQ
CSB-EP746045EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Urease accessory protein UreE (ureE) CSB-YP746046EYQ
CSB-EP746046EYQ
CSB-BP746046EYQ
CSB-MP746046EYQ
CSB-EP746046EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP746047EYQ
CSB-EP746047EYQ
CSB-BP746047EYQ
CSB-MP746047EYQ
CSB-EP746047EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Leucine--tRNA ligase (leuS), partial CSB-YP746048EYQ
CSB-EP746048EYQ
CSB-BP746048EYQ
CSB-MP746048EYQ
CSB-EP746048EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Glutamate 5-kinase (proB) CSB-YP746049EYQ
CSB-EP746049EYQ
CSB-BP746049EYQ
CSB-MP746049EYQ
CSB-EP746049EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Cytochrome c biogenesis protein CcsA (ccsA), partial CSB-YP746050EYQ
CSB-EP746050EYQ
CSB-BP746050EYQ
CSB-MP746050EYQ
CSB-EP746050EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP746051EYQ
CSB-EP746051EYQ
CSB-BP746051EYQ
CSB-MP746051EYQ
CSB-EP746051EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Tryptophan--tRNA ligase (trpS) CSB-YP746052EYQ
CSB-EP746052EYQ
CSB-BP746052EYQ
CSB-MP746052EYQ
CSB-EP746052EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris ATP phosphoribosyltransferase (hisG) CSB-YP746053EYQ
CSB-EP746053EYQ
CSB-BP746053EYQ
CSB-MP746053EYQ
CSB-EP746053EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP746054EYQ
CSB-EP746054EYQ
CSB-BP746054EYQ
CSB-MP746054EYQ
CSB-EP746054EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L19 (rplS) CSB-YP746055EYQ
CSB-EP746055EYQ
CSB-BP746055EYQ
CSB-MP746055EYQ
CSB-EP746055EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP746056EYQ
CSB-EP746056EYQ
CSB-BP746056EYQ
CSB-MP746056EYQ
CSB-EP746056EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE), partial CSB-YP746057EYQ
CSB-EP746057EYQ
CSB-BP746057EYQ
CSB-MP746057EYQ
CSB-EP746057EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem I reaction center subunit IV (psaE) CSB-YP746058EYQ
CSB-EP746058EYQ
CSB-BP746058EYQ
CSB-MP746058EYQ
CSB-EP746058EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris S-adenosylmethionine synthase (metK) CSB-YP746059EYQ
CSB-EP746059EYQ
CSB-BP746059EYQ
CSB-MP746059EYQ
CSB-EP746059EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris NAD (P)H-quinone oxidoreductase subunit J (ndhJ) CSB-YP746060EYQ
CSB-EP746060EYQ
CSB-BP746060EYQ
CSB-MP746060EYQ
CSB-EP746060EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem II reaction center protein I (psbI), partial CSB-YP746061EYQ
CSB-EP746061EYQ
CSB-BP746061EYQ
CSB-MP746061EYQ
CSB-EP746061EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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