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  • Your Good Partner in Biology Research

    重組蛋白

    產(chǎn)品名稱 貨號 規(guī)格
    Recombinant Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP745982SZB
    CSB-EP745982SZB
    CSB-BP745982SZB
    CSB-MP745982SZB
    CSB-EP745982SZB-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP745983SZB
    CSB-EP745983SZB
    CSB-BP745983SZB
    CSB-MP745983SZB
    CSB-EP745983SZB-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant UPF0114 protein YqhA (yqhA), partial CSB-YP745984SZB1
    CSB-EP745984SZB1
    CSB-BP745984SZB1
    CSB-MP745984SZB1
    CSB-EP745984SZB1-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant tRNA-dihydrouridine synthase C (dusC) CSB-YP745985SZB
    CSB-EP745985SZB
    CSB-BP745985SZB
    CSB-MP745985SZB
    CSB-EP745985SZB-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Septum site-determining protein MinC (minC) CSB-YP745986SZB
    CSB-EP745986SZB
    CSB-BP745986SZB
    CSB-MP745986SZB
    CSB-EP745986SZB-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Probable 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase (citG) CSB-YP745987SZB
    CSB-EP745987SZB
    CSB-BP745987SZB
    CSB-MP745987SZB
    CSB-EP745987SZB-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Protein translocase subunit SecA 1 (secA1), partial CSB-YP745988RDR
    CSB-EP745988RDR
    CSB-BP745988RDR
    CSB-MP745988RDR
    CSB-EP745988RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Cytidylate kinase (cmk) CSB-YP745989RDR
    CSB-EP745989RDR
    CSB-BP745989RDR
    CSB-MP745989RDR
    CSB-EP745989RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Biotin synthase (bioB) CSB-YP745990RDR
    CSB-EP745990RDR
    CSB-BP745990RDR
    CSB-MP745990RDR
    CSB-EP745990RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica ATP synthase subunit b 2 (atpF2), partial CSB-YP745991RDR
    CSB-EP745991RDR
    CSB-BP745991RDR
    CSB-MP745991RDR
    CSB-EP745991RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP745992RDR
    CSB-EP745992RDR
    CSB-BP745992RDR
    CSB-MP745992RDR
    CSB-EP745992RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Aspartate--tRNA ligase (aspS), partial CSB-YP745993RDR
    CSB-EP745993RDR
    CSB-BP745993RDR
    CSB-MP745993RDR
    CSB-EP745993RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Transcriptional repressor NrdR (nrdR) CSB-YP745994RDR
    CSB-EP745994RDR
    CSB-BP745994RDR
    CSB-MP745994RDR
    CSB-EP745994RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Ribonuclease 3 (rnc) CSB-YP745995RDR
    CSB-EP745995RDR
    CSB-BP745995RDR
    CSB-MP745995RDR
    CSB-EP745995RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP745996RDR
    CSB-EP745996RDR
    CSB-BP745996RDR
    CSB-MP745996RDR
    CSB-EP745996RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Vitamin B12-dependent ribonucleotide reductase (nrdJ), partial CSB-YP745997RDR
    CSB-EP745997RDR
    CSB-BP745997RDR
    CSB-MP745997RDR
    CSB-EP745997RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica 30S ribosomal protein S13 (rpsM) CSB-YP745998RDR
    CSB-EP745998RDR
    CSB-BP745998RDR
    CSB-MP745998RDR
    CSB-EP745998RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP745999RDR
    CSB-EP745999RDR
    CSB-BP745999RDR
    CSB-MP745999RDR
    CSB-EP745999RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP746000RDR
    CSB-EP746000RDR
    CSB-BP746000RDR
    CSB-MP746000RDR
    CSB-EP746000RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)
    Recombinant Rhodopirellula baltica Protein RecA (recA) CSB-YP746001RDR
    CSB-EP746001RDR
    CSB-BP746001RDR
    CSB-MP746001RDR
    CSB-EP746001RDR-B
    20μg/100μg/1mg(1mg*1 or 500ug*2)

    有用資源

    什么是重組蛋白?

    重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

    第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

    第二條:如何選擇合適的表達(dá)載體?

    表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

    第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

    根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

    引用資料

    Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

    Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

    Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

    Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

    Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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