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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus 50S ribosomal protein L36 (rpmJ) CSB-YP746582SKW
CSB-EP746582SKW
CSB-BP746582SKW
CSB-MP746582SKW
CSB-EP746582SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L17 (rplQ) CSB-YP746583SKW
CSB-EP746583SKW
CSB-BP746583SKW
CSB-MP746583SKW
CSB-EP746583SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus NAD-dependent protein deacetylase (cobB) CSB-YP746584SKW
CSB-EP746584SKW
CSB-BP746584SKW
CSB-MP746584SKW
CSB-EP746584SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Zinc-type alcohol dehydrogenase-like protein SAS2087 (SAS2087) CSB-YP746585SKW
CSB-EP746585SKW
CSB-BP746585SKW
CSB-MP746585SKW
CSB-EP746585SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoglucosamine mutase (glmM) CSB-YP746586SKW
CSB-EP746586SKW
CSB-BP746586SKW
CSB-MP746586SKW
CSB-EP746586SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Low molecular weight protein-tyrosine-phosphatase ptpB (ptpB) CSB-YP746587SKW
CSB-EP746587SKW
CSB-BP746587SKW
CSB-MP746587SKW
CSB-EP746587SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable transglycosylase sceD (sceD) CSB-YP746588SKW
CSB-EP746588SKW
CSB-BP746588SKW
CSB-MP746588SKW
CSB-EP746588SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cardiolipin synthase (cls), partial CSB-YP746589SKW1
CSB-EP746589SKW1
CSB-BP746589SKW1
CSB-MP746589SKW1
CSB-EP746589SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alanine racemase 1 (alr1) CSB-YP746590SKW
CSB-EP746590SKW
CSB-BP746590SKW
CSB-MP746590SKW
CSB-EP746590SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Delta-hemolysin (hld) CSB-YP746591SKW
CSB-EP746591SKW
CSB-BP746591SKW
CSB-MP746591SKW
CSB-EP746591SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA ligase (ligA), partial CSB-YP746592SKW
CSB-EP746592SKW
CSB-BP746592SKW
CSB-MP746592SKW
CSB-EP746592SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Diacylglycerol kinase (dagK) CSB-YP746593SKW
CSB-EP746593SKW
CSB-BP746593SKW
CSB-MP746593SKW
CSB-EP746593SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0435 protein SAS1802 (SAS1802) CSB-YP746594SKW
CSB-EP746594SKW
CSB-BP746594SKW
CSB-MP746594SKW
CSB-EP746594SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0342 protein SAS1766 (SAS1766) CSB-YP746595SKW
CSB-EP746595SKW
CSB-BP746595SKW
CSB-MP746595SKW
CSB-EP746595SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-adenosylmethionine synthase (metK) CSB-YP746596SKW
CSB-EP746596SKW
CSB-BP746596SKW
CSB-MP746596SKW
CSB-EP746596SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-alanine aminotransferase (dat) CSB-YP746597SKW
CSB-EP746597SKW
CSB-BP746597SKW
CSB-MP746597SKW
CSB-EP746597SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein H (isdH), partial CSB-YP746598SKW
CSB-EP746598SKW
CSB-BP746598SKW
CSB-MP746598SKW
CSB-EP746598SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Septation ring formation regulator EzrA (ezrA), partial CSB-YP746599SKW
CSB-EP746599SKW
CSB-BP746599SKW
CSB-MP746599SKW
CSB-EP746599SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP746600SKW
CSB-EP746600SKW
CSB-BP746600SKW
CSB-MP746600SKW
CSB-EP746600SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Threonine--tRNA ligase (thrS), partial CSB-YP746601SKW
CSB-EP746601SKW
CSB-BP746601SKW
CSB-MP746601SKW
CSB-EP746601SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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