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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Signal peptidase IB (spsB), partial CSB-YP746642SKW
CSB-EP746642SKW
CSB-BP746642SKW
CSB-MP746642SKW
CSB-EP746642SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative peptidyl-prolyl cis-trans isomerase (SAS0824) CSB-YP746643SKW
CSB-EP746643SKW
CSB-BP746643SKW
CSB-MP746643SKW
CSB-EP746643SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit F1 (mnhF1), partial CSB-YP746644SKW1
CSB-EP746644SKW1
CSB-BP746644SKW1
CSB-MP746644SKW1
CSB-EP746644SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lipoyl synthase (lipA) CSB-YP746645SKW
CSB-EP746645SKW
CSB-BP746645SKW
CSB-MP746645SKW
CSB-EP746645SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP746646SKW
CSB-EP746646SKW
CSB-BP746646SKW
CSB-MP746646SKW
CSB-EP746646SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP746647SKW
CSB-EP746647SKW
CSB-BP746647SKW
CSB-MP746647SKW
CSB-EP746647SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0042 nucleotide-binding protein SAS0730 (SAS0730) CSB-YP746648SKW
CSB-EP746648SKW
CSB-BP746648SKW
CSB-MP746648SKW
CSB-EP746648SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein SAS0717 (SAS0717) CSB-YP746649SKW
CSB-EP746649SKW
CSB-BP746649SKW
CSB-MP746649SKW
CSB-EP746649SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Quinolone resistance protein norA (norA), partial CSB-YP746650SKW
CSB-EP746650SKW
CSB-BP746650SKW
CSB-MP746650SKW
CSB-EP746650SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Sensor histidine kinase graS (graS), partial CSB-YP746651SKW1
CSB-EP746651SKW1
CSB-BP746651SKW1
CSB-MP746651SKW1
CSB-EP746651SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alcohol dehydrogenase (adh) CSB-YP746652SKW
CSB-EP746652SKW
CSB-BP746652SKW
CSB-MP746652SKW
CSB-EP746652SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative long chain fatty acid-CoA ligase VraA (vraA) CSB-YP746653SKW
CSB-EP746653SKW
CSB-BP746653SKW
CSB-MP746653SKW
CSB-EP746653SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine-aspartate repeat-containing protein D (sdrD), partial CSB-YP746654SKW
CSB-EP746654SKW
CSB-BP746654SKW
CSB-MP746654SKW
CSB-EP746654SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molecular chaperone Hsp31 and glyoxalase 3 (hchA) CSB-YP746655SKW
CSB-EP746655SKW
CSB-BP746655SKW
CSB-MP746655SKW
CSB-EP746655SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP746656SKW
CSB-EP746656SKW
CSB-BP746656SKW
CSB-MP746656SKW
CSB-EP746656SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L11 (rplK) CSB-YP746657SKW
CSB-EP746657SKW
CSB-BP746657SKW
CSB-MP746657SKW
CSB-EP746657SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ATP:guanido phosphotransferase SAS0481 (SAS0481) CSB-YP746658SKW
CSB-EP746658SKW
CSB-BP746658SKW
CSB-MP746658SKW
CSB-EP746658SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative septation protein spoVG (spoVG) CSB-YP746659SKW
CSB-EP746659SKW
CSB-BP746659SKW
CSB-MP746659SKW
CSB-EP746659SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Recombination protein RecR (recR) CSB-YP746660SKW
CSB-EP746660SKW
CSB-BP746660SKW
CSB-MP746660SKW
CSB-EP746660SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0753 protein SAS0411 (SAS0411), partial CSB-YP746661SKW
CSB-EP746661SKW
CSB-BP746661SKW
CSB-MP746661SKW
CSB-EP746661SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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