Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Postreplication repair E3 ubiquitin-protein ligase RAD18 (RAD18) CSB-YP748424DOT
CSB-EP748424DOT
CSB-BP748424DOT
CSB-MP748424DOT
CSB-EP748424DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DASH complex subunit DAM1 (DAM1) CSB-YP748425DOT
CSB-EP748425DOT
CSB-BP748425DOT
CSB-MP748425DOT
CSB-EP748425DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Inositol-pentakisphosphate 2-kinase (IPK1) CSB-YP748426DOT
CSB-EP748426DOT
CSB-BP748426DOT
CSB-MP748426DOT
CSB-EP748426DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial homologous recombination protein 1 (MHR1) CSB-YP748427DOT
CSB-EP748427DOT
CSB-BP748427DOT
CSB-MP748427DOT
CSB-EP748427DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 26S proteasome regulatory subunit RPN8 (RPN8) CSB-YP748428DOT
CSB-EP748428DOT
CSB-BP748428DOT
CSB-MP748428DOT
CSB-EP748428DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ADIPOR-like receptor IZH3 (IZH3), partial CSB-YP748429DOT1
CSB-EP748429DOT1
CSB-BP748429DOT1
CSB-MP748429DOT1
CSB-EP748429DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Demethylmenaquinone methyltransferase (ubiE) CSB-YP748430LEV
CSB-EP748430LEV
CSB-BP748430LEV
CSB-MP748430LEV
CSB-EP748430LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Ribonuclease 3 (rnc) CSB-YP748431LEV
CSB-EP748431LEV
CSB-BP748431LEV
CSB-MP748431LEV
CSB-EP748431LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica BURP domain-containing protein 8 (BURP8) CSB-YP748432OFG
CSB-EP748432OFG
CSB-BP748432OFG
CSB-MP748432OFG
CSB-EP748432OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica CBL-interacting protein kinase 7 (CIPK7) CSB-YP748433OFG
CSB-EP748433OFG
CSB-BP748433OFG
CSB-MP748433OFG
CSB-EP748433OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Fe (2+) transport protein 1 (IRT1), partial CSB-YP748434OFG
CSB-EP748434OFG
CSB-BP748434OFG
CSB-MP748434OFG
CSB-EP748434OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Tubby-like F-box protein 8 (TULP8) CSB-YP748435OFG
CSB-EP748435OFG
CSB-BP748435OFG
CSB-MP748435OFG
CSB-EP748435OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica ATP-dependent DNA helicase 2 subunit KU80 (KU80), partial CSB-YP748436OFG
CSB-EP748436OFG
CSB-BP748436OFG
CSB-MP748436OFG
CSB-EP748436OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum UPF0136 membrane protein (DDB_G0271790), partial CSB-YP748437DKK1
CSB-EP748437DKK1
CSB-BP748437DKK1
CSB-MP748437DKK1
CSB-EP748437DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP748438DKK
CSB-EP748438DKK
CSB-BP748438DKK
CSB-MP748438DKK
CSB-EP748438DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Dual specificity protein kinase zakA (zakA), partial CSB-YP748439DKK
CSB-EP748439DKK
CSB-BP748439DKK
CSB-MP748439DKK
CSB-EP748439DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Calponin homology domain-containing protein DDB_G0272472 (DDB_G0272472), partial CSB-YP748440DKK
CSB-EP748440DKK
CSB-BP748440DKK
CSB-MP748440DKK
CSB-EP748440DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Isocitrate dehydrogenase [NADP], mitochondrial (idhM) CSB-YP748441DKK
CSB-EP748441DKK
CSB-BP748441DKK
CSB-MP748441DKK
CSB-EP748441DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum GATA zinc finger domain-containing protein 8 (gtaH), partial CSB-YP748442DKK
CSB-EP748442DKK
CSB-BP748442DKK
CSB-MP748442DKK
CSB-EP748442DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger CCCH domain-containing protein 36 (Os05g0497500, LOC_Os05g41790), partial CSB-YP748443OFG
CSB-EP748443OFG
CSB-BP748443OFG
CSB-MP748443OFG
CSB-EP748443OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>