Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Charged multivesicular body protein 6-A (chmp6-a) CSB-YP750186XBE
CSB-EP750186XBE
CSB-BP750186XBE
CSB-MP750186XBE
CSB-EP750186XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Riboflavin transporter 2-B (rft2b), partial CSB-YP750187DIL
CSB-EP750187DIL
CSB-BP750187DIL
CSB-MP750187DIL
CSB-EP750187DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UDP-glucuronic acid decarboxylase 1 (uxs1), partial CSB-YP750188DIL
CSB-EP750188DIL
CSB-BP750188DIL
CSB-MP750188DIL
CSB-EP750188DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytidine monophosphate-N-acetylneuraminic acid hydroxylase (cmah), partial CSB-YP750189DIL
CSB-EP750189DIL
CSB-BP750189DIL
CSB-MP750189DIL
CSB-EP750189DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histone-lysine N-trimethyltransferase SMYD5 (SMYD5) CSB-YP750190HU
CSB-EP750190HU
CSB-BP750190HU
CSB-MP750190HU
CSB-EP750190HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Phosphatidylinositol 4-kinase beta (pi4kb), partial CSB-YP750191XBE
CSB-EP750191XBE
CSB-BP750191XBE
CSB-MP750191XBE
CSB-EP750191XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis N-lysine methyltransferase SMYD2-B (smyd2-b) CSB-YP750192XBE
CSB-EP750192XBE
CSB-BP750192XBE
CSB-MP750192XBE
CSB-EP750192XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 5'-nucleotidase domain-containing protein 3 (nt5dc3) CSB-YP750193XBE
CSB-EP750193XBE
CSB-BP750193XBE
CSB-MP750193XBE
CSB-EP750193XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ubiquitin carboxyl-terminal hydrolase 22-A (usp22-a), partial CSB-YP750194XBE
CSB-EP750194XBE
CSB-BP750194XBE
CSB-MP750194XBE
CSB-EP750194XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Coenzyme Q-binding protein COQ10 homolog B, mitochondrial (coq10b), partial CSB-YP750195XBE
CSB-EP750195XBE
CSB-BP750195XBE
CSB-MP750195XBE
CSB-EP750195XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Lysosomal thioesterase PPT2-B (ppt2-b) CSB-YP750196XBE
CSB-EP750196XBE
CSB-BP750196XBE
CSB-MP750196XBE
CSB-EP750196XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Suppressor of IKBKE 1 (sike1) CSB-YP750197XBE
CSB-EP750197XBE
CSB-BP750197XBE
CSB-MP750197XBE
CSB-EP750197XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0197 transmembrane protein C11orf10 homolog, partial CSB-YP750198XBE1
CSB-EP750198XBE1
CSB-BP750198XBE1
CSB-MP750198XBE1
CSB-EP750198XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein tweety homolog 3 (ttyh3), partial CSB-YP750199XBE1
CSB-EP750199XBE1
CSB-BP750199XBE1
CSB-MP750199XBE1
CSB-EP750199XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Rho GTPase-activating protein 32 (arhgap32), partial CSB-YP750200XBE
CSB-EP750200XBE
CSB-BP750200XBE
CSB-MP750200XBE
CSB-EP750200XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cullin-3-B (cul3b), partial CSB-YP750201XBE
CSB-EP750201XBE
CSB-BP750201XBE
CSB-MP750201XBE
CSB-EP750201XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis RNA methyltransferase-like protein 1 (rnmtl1) CSB-YP750202XBE
CSB-EP750202XBE
CSB-BP750202XBE
CSB-MP750202XBE
CSB-EP750202XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Aurora kinase B-B (aurkb-b) CSB-YP750203XBE
CSB-EP750203XBE
CSB-BP750203XBE
CSB-MP750203XBE
CSB-EP750203XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Probable 39S ribosomal protein L24, mitochondrial (mrpl24) CSB-YP750204XBE
CSB-EP750204XBE
CSB-BP750204XBE
CSB-MP750204XBE
CSB-EP750204XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Endothelial differentiation-related factor 1 homolog (edf1) CSB-YP750205XBE
CSB-EP750205XBE
CSB-BP750205XBE
CSB-MP750205XBE
CSB-EP750205XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>