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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Sensor protein vraS (vraS), partial CSB-YP749986SKW1
CSB-EP749986SKW1
CSB-BP749986SKW1
CSB-MP749986SKW1
CSB-EP749986SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate-1-semialdehyde 2,1-aminomutase 2 (hemL2) CSB-YP749987SKW
CSB-EP749987SKW
CSB-BP749987SKW
CSB-MP749987SKW
CSB-EP749987SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0754 membrane protein SAS1767 (SAS1767), partial CSB-YP749988SKW1
CSB-EP749988SKW1
CSB-BP749988SKW1
CSB-MP749988SKW1
CSB-EP749988SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splA (splA) CSB-YP749989SKW
CSB-EP749989SKW
CSB-BP749989SKW
CSB-MP749989SKW
CSB-EP749989SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus RNA polymerase sigma factor sigS (sigS) CSB-YP749990SKW
CSB-EP749990SKW
CSB-BP749990SKW
CSB-MP749990SKW
CSB-EP749990SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized peptidase SAS1635 (SAS1635) CSB-YP749991SKW
CSB-EP749991SKW
CSB-BP749991SKW
CSB-MP749991SKW
CSB-EP749991SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dephospho-CoA kinase (coaE) CSB-YP749992SKW
CSB-EP749992SKW
CSB-BP749992SKW
CSB-MP749992SKW
CSB-EP749992SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Translation initiation factor IF-3 (infC) CSB-YP749993SKW
CSB-EP749993SKW
CSB-BP749993SKW
CSB-MP749993SKW
CSB-EP749993SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase obg (obg) CSB-YP749994SKW
CSB-EP749994SKW
CSB-BP749994SKW
CSB-MP749994SKW
CSB-EP749994SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0337 protein SAS1561 (SAS1561) CSB-YP749995SKW
CSB-EP749995SKW
CSB-BP749995SKW
CSB-MP749995SKW
CSB-EP749995SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor 4 (lepA), partial CSB-YP749996SKW
CSB-EP749996SKW
CSB-BP749996SKW
CSB-MP749996SKW
CSB-EP749996SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chaperone protein DnaJ (dnaJ) CSB-YP749997SKW
CSB-EP749997SKW
CSB-BP749997SKW
CSB-MP749997SKW
CSB-EP749997SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA repair protein recO (recO) CSB-YP749998SKW
CSB-EP749998SKW
CSB-BP749998SKW
CSB-MP749998SKW
CSB-EP749998SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Superoxide dismutase [Mn/Fe] 1 (sodA) CSB-YP749999SKW
CSB-EP749999SKW
CSB-BP749999SKW
CSB-MP749999SKW
CSB-EP749999SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor P (efp) CSB-YP750000SKW
CSB-EP750000SKW
CSB-BP750000SKW
CSB-MP750000SKW
CSB-EP750000SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine recombinase XerD (xerD) CSB-YP750001SKW
CSB-EP750001SKW
CSB-BP750001SKW
CSB-MP750001SKW
CSB-EP750001SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP750002SKW
CSB-EP750002SKW
CSB-BP750002SKW
CSB-MP750002SKW
CSB-EP750002SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP750003SKW
CSB-EP750003SKW
CSB-BP750003SKW
CSB-MP750003SKW
CSB-EP750003SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Signal transduction histidine-protein kinase ArlS (arlS), partial CSB-YP750004SKW1
CSB-EP750004SKW1
CSB-BP750004SKW1
CSB-MP750004SKW1
CSB-EP750004SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (odhB) CSB-YP750005SKW
CSB-EP750005SKW
CSB-BP750005SKW
CSB-MP750005SKW
CSB-EP750005SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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