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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Haemophilus ducreyi UPF0102 protein HD_0802 (HD_0802) CSB-YP749625HSY
CSB-EP749625HSY
CSB-BP749625HSY
CSB-MP749625HSY
CSB-EP749625HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 50S ribosomal protein L32 (rpmF) CSB-YP749626HSY
CSB-EP749626HSY
CSB-BP749626HSY
CSB-MP749626HSY
CSB-EP749626HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Protein hfq (hfq) CSB-YP749627HSY
CSB-EP749627HSY
CSB-BP749627HSY
CSB-MP749627HSY
CSB-EP749627HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Sec-independent protein translocase protein TatA (tatA), partial CSB-YP749628HSY
CSB-EP749628HSY
CSB-BP749628HSY
CSB-MP749628HSY
CSB-EP749628HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Trigger factor (tig) CSB-YP749629HSY
CSB-EP749629HSY
CSB-BP749629HSY
CSB-MP749629HSY
CSB-EP749629HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP749630HSY
CSB-EP749630HSY
CSB-BP749630HSY
CSB-MP749630HSY
CSB-EP749630HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP749631HSY
CSB-EP749631HSY
CSB-BP749631HSY
CSB-MP749631HSY
CSB-EP749631HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi DNA mismatch repair protein mutH (mutH) CSB-YP749632HSY
CSB-EP749632HSY
CSB-BP749632HSY
CSB-MP749632HSY
CSB-EP749632HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi tRNA-dihydrouridine synthase B (dusB) CSB-YP749633HSY
CSB-EP749633HSY
CSB-BP749633HSY
CSB-MP749633HSY
CSB-EP749633HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Protein RecA (recA) CSB-YP749634HSY
CSB-EP749634HSY
CSB-BP749634HSY
CSB-MP749634HSY
CSB-EP749634HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Na (+)-translocating NADH-quinone reductase subunit C (nqrC), partial CSB-YP749635HSY1
CSB-EP749635HSY1
CSB-BP749635HSY1
CSB-MP749635HSY1
CSB-EP749635HSY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Translation initiation factor IF-1 (infA) CSB-YP749636HSY
CSB-EP749636HSY
CSB-BP749636HSY
CSB-MP749636HSY
CSB-EP749636HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP749637HSY
CSB-EP749637HSY
CSB-BP749637HSY
CSB-MP749637HSY
CSB-EP749637HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Ribonuclease PH (rph) CSB-YP749638HSY
CSB-EP749638HSY
CSB-BP749638HSY
CSB-MP749638HSY
CSB-EP749638HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Lipid II flippase FtsW (ftsW), partial CSB-YP749639HSY
CSB-EP749639HSY
CSB-BP749639HSY
CSB-MP749639HSY
CSB-EP749639HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP749640HSY
CSB-EP749640HSY
CSB-BP749640HSY
CSB-MP749640HSY
CSB-EP749640HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 50S ribosomal protein L21 (rplU) CSB-YP749641HSY
CSB-EP749641HSY
CSB-BP749641HSY
CSB-MP749641HSY
CSB-EP749641HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Inorganic pyrophosphatase (ppa) CSB-YP749642HSY
CSB-EP749642HSY
CSB-BP749642HSY
CSB-MP749642HSY
CSB-EP749642HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi UPF0350 protein HD_0065 (HD_0065) CSB-YP749643HSY
CSB-EP749643HSY
CSB-BP749643HSY
CSB-MP749643HSY
CSB-EP749643HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Diaminopimelate epimerase (dapF) CSB-YP749644HSY
CSB-EP749644HSY
CSB-BP749644HSY
CSB-MP749644HSY
CSB-EP749644HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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