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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella pertussis Heat-inducible transcription repressor HrcA (hrcA) CSB-YP749705BUA
CSB-EP749705BUA
CSB-BP749705BUA
CSB-MP749705BUA
CSB-EP749705BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Serine--tRNA ligase (serS) CSB-YP749706BUA
CSB-EP749706BUA
CSB-BP749706BUA
CSB-MP749706BUA
CSB-EP749706BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Probable cytosol aminopeptidase (pepA) CSB-YP749707BUA
CSB-EP749707BUA
CSB-BP749707BUA
CSB-MP749707BUA
CSB-EP749707BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP749708BUA1
CSB-EP749708BUA1
CSB-BP749708BUA1
CSB-MP749708BUA1
CSB-EP749708BUA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Nucleoside diphosphate kinase (ndk) CSB-YP749709BUA
CSB-EP749709BUA
CSB-BP749709BUA
CSB-MP749709BUA
CSB-EP749709BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Adenylosuccinate synthetase (purA) CSB-YP749710BUA
CSB-EP749710BUA
CSB-BP749710BUA
CSB-MP749710BUA
CSB-EP749710BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 50S ribosomal protein L28 (rpmB) CSB-YP749711BUA
CSB-EP749711BUA
CSB-BP749711BUA
CSB-MP749711BUA
CSB-EP749711BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP749712BUA
CSB-EP749712BUA
CSB-BP749712BUA
CSB-MP749712BUA
CSB-EP749712BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Alanine--tRNA ligase (alaS), partial CSB-YP749713BUA
CSB-EP749713BUA
CSB-BP749713BUA
CSB-MP749713BUA
CSB-EP749713BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Trigger factor (tig) CSB-YP749714BUA
CSB-EP749714BUA
CSB-BP749714BUA
CSB-MP749714BUA
CSB-EP749714BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD (rlmD) CSB-YP749715BUA
CSB-EP749715BUA
CSB-BP749715BUA
CSB-MP749715BUA
CSB-EP749715BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Dihydrodipicolinate synthase (dapA) CSB-YP749716BUA
CSB-EP749716BUA
CSB-BP749716BUA
CSB-MP749716BUA
CSB-EP749716BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP749717BUA
CSB-EP749717BUA
CSB-BP749717BUA
CSB-MP749717BUA
CSB-EP749717BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis SsrA-binding protein (smpB) CSB-YP749718BUA
CSB-EP749718BUA
CSB-BP749718BUA
CSB-MP749718BUA
CSB-EP749718BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Ribosome-recycling factor (frr) CSB-YP749719BUA
CSB-EP749719BUA
CSB-BP749719BUA
CSB-MP749719BUA
CSB-EP749719BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP749720BUA
CSB-EP749720BUA
CSB-BP749720BUA
CSB-MP749720BUA
CSB-EP749720BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP749721BUA
CSB-EP749721BUA
CSB-BP749721BUA
CSB-MP749721BUA
CSB-EP749721BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Tryptophan--tRNA ligase (trpS) CSB-YP749722BUA
CSB-EP749722BUA
CSB-BP749722BUA
CSB-MP749722BUA
CSB-EP749722BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Phosphoglucosamine mutase (glmM) CSB-YP749723BUA
CSB-EP749723BUA
CSB-BP749723BUA
CSB-MP749723BUA
CSB-EP749723BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Protein phosphatase CheZ (cheZ) CSB-YP749724BUA
CSB-EP749724BUA
CSB-BP749724BUA
CSB-MP749724BUA
CSB-EP749724BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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